A resource recovery from waste material is an important topic. Microbial fuel cell (MFC) is getting interest nowadays due to its superiority to generate electricity from a wide range of substrates by utilizing organic wastes. This study was aimed to investigate the potential electricity generation by utilizing animal dung and leaf mold as a very prospective substrate for bioelectricity generation using MFC. MFC as a direct current source (DC), in this experiment we focus to prove the potential difference (voltage) generated by the material. The best result is 10980 ± 11.32 mV/m² for a mixed sample of Test V (leaf mold and soil), while for the pure samples showed at 10880 ± 4.784 mV/m² for Test IV (chicken dropping). The advancements in this environmentally friendly study have expanded that cow dung, chicken droppings and leaf mold as a variety of organic wastes which contains large amounts of nutrients and various other minerals, as a microbial fuel cell proved to generate sustainable green and safe electricity moreover as an efficient, eco-friendly solution for organic waste management.