In Vitro Evaluation of Anticancer Drugs with Kinetic and Static Alternating Cell Culture System - Journal of Cancer Therapy

JCT > Vol.8 No.9, September 2017

Journal of Cancer Therapy

Volume 8, Issue 9 (September 2017)

ISSN Print: 2151-1934 ISSN Online: 2151-1942

Google-based Impact Factor: 0.30 Citations h5-index & Ranking

In Vitro Evaluation of Anticancer Drugs with Kinetic and Static Alternating Cell Culture System ()

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Download as PDF (Size: 490KB) PP. 845-859

DOI: 10.4236/jct.2017.89074 1,036 Downloads 2,009 Views Citations

Author(s)

Yongxin Zhang^1*, Ying Wang¹, Zhenying Wang¹, Farhang Farhangfar², Monica Zimmerman¹

Affiliation(s)

¹Zyxell Inc., Carrollton, TX, USA.
²Carolinas Healthcare System, Charlotte, NC, USA.

ABSTRACT

Variable bioreactors have been developed for the evaluation of anti-cancer drug efficacy. The Kinetic and Static Alternating Cell Culture System (KSACCS) combines the advantages of kinetic bioreactors and static cultures to improve cell growth by providing adequate metabolic support while minimizing shear-stress. In the current studies, the KSACCS in the ZYX Bioreactor could significantly increase the sensitivity of lung cancer cells (PLS008) and leukemia cells (HL60) to anticancer drugs Cisplatin and 5-FU by accelerating the apoptosis of cancer cells. It was also shown that excessive agitation of the cells could lead to severe cell damage, which resulted in a diminished sensitivity of anticancer drug evaluation, and co-culture systems tend to reduce the sensitivity of anticancer drug evaluation although it might better mimic in vivo conditions.

KEYWORDS

Anticancer Drug Evaluation, Cell Culture, Bioreactor

Share and Cite:

Zhang, Y. , Wang, Y. , Wang, Z. , Farhangfar, F. and Zimmerman, M. (2017) In Vitro Evaluation of Anticancer Drugs with Kinetic and Static Alternating Cell Culture System. Journal of Cancer Therapy, 8, 845-859. doi: 10.4236/jct.2017.89074.

Cited by

[1]	Inhibition of cancer cell immune evasion by combined application of cytotoxic T-lymphocytes and natural killer cells
	2018

[2]	Improvement of cancer stem cell expansion with kinetic and static alternating culture system
	2018

[3]	Application of Bioreactor in Stem Cell Culture
	2017

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