Enzymatic Synthesis and Structural Characterization of Rebaudioside D3, a Minor Steviol Glycoside of Stevia rebaudiana Bertoni - American Journal of Plant Sciences

AJPS > Vol.8 No.3, February 2017

Volume 8, Issue 3 (February 2017)

ISSN Print: 2158-2742 ISSN Online: 2158-2750

Google-based Impact Factor: 1.20 Citations h5-index & Ranking

Enzymatic Synthesis and Structural Characterization of Rebaudioside D3, a Minor Steviol Glycoside of Stevia rebaudiana Bertoni ()

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DOI: 10.4236/ajps.2017.83030 1,920 Downloads 3,939 Views Citations

Author(s)

Guohong Mao^1*, Venkata Sai Prakash Chaturvedula², Jacob Edward Vick¹, Oliver Yu^1,3

Affiliation(s)

¹Conagen Inc., Bedford, MA, USA.
²Blue California, Rancho Santa Margarita, CA, USA.
³Wuxi New Way Biotechnology Ltd., Wuxi, China.

ABSTRACT

Rebaudioside D3, a novel steviol glycoside, is produced by specific UDP-glycosyltransferase of rebaudioside E, a minor steviol glycoside of Stevia rebaudiana Bertoni. The complete proton and carbon NMR spectral assignments of rebaudioside D3, 13-[(2-O-β-D-glucopyranosyl-6-O-β-D-glucopyranosyl-β-D-glucopyranosyl) oxy] ent-kaur-16-en-19-oic acid-(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl) ester, was achieved by the extensive 1D and 2D NMR (¹H and ¹³C, TOCSY, HMQC, HMBC) as well as mass spectral data. Further, hydrolysis studies were performed on rebaudioside D3 using acid and enzymatic studies to identify aglycone and sugar residues in its structure. Rebaudioside D3 is detected in the commercial extract of the leaves of Stevia rebaudiana by LC-MS analysis, suggesting rebaudioside D3 is a natural steviol glycoside.

KEYWORDS

Stevia rebaudiana, Diterpene Glycoside, Structure Characterization, Spectral Data, LC-MS

Share and Cite:

Mao, G. , Chaturvedula, V. , Vick, J. and Yu, O. (2017) Enzymatic Synthesis and Structural Characterization of Rebaudioside D3, a Minor Steviol Glycoside of Stevia rebaudiana Bertoni. American Journal of Plant Sciences, 8, 441-450. doi: 10.4236/ajps.2017.83030.

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