The Biomass Dosage Influences the Effects of Diethyl Aminoethyl Hexanoate on Micropropagation of Echinacea purpurea (L.) Moench - Journal of Biosciences and Medicines

JBM > Vol.4 No.12, December 2016

Volume 4, Issue 12 (December 2016)

ISSN Print: 2327-5081 ISSN Online: 2327-509X

Google-based Impact Factor: 0.51 Citations

The Biomass Dosage Influences the Effects of Diethyl Aminoethyl Hexanoate on Micropropagation of Echinacea purpurea (L.) Moench ()

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DOI: 10.4236/jbm.2016.412014 1,421 Downloads 3,017 Views Citations

Author(s)

Xiaolu Chen^1,2*, Dongliang Li³, Junjie Zhang², Qingling Li², Yuesheng Yang^2*, Hong Wu²

Affiliation(s)

¹Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Danzhou, China.
²College of Life Science, South China Agricultural University, Guangzhou, China.
³Institute of Tropical Horticulture Research, Hainan Academy of Agricultural Science, Haikou, China.

ABSTRACT

The plant growth regulator diethyl aminoethyl hexanoate (DA-6) has proved highly effective on micropropagation of the medicinal plant purple coneflower (Echinacea purpurea (L.) Moench), however, sharp variation of the effects existed among explants in the same treatment, making the application of DA-6 in micropropagation difficult. In order to clarify factors that influencing the treating results of DA-6, explants with different biomass dosage were prepared and inoculated onto medium supplemented with different concentrations of DA-6. It was found that among the three kinds of biomass dosage explants, the lowest biomass explants required the lowest concentration of DA-6, and the highest biomass explants required the highest concentration of DA-6 for the best results on adventitious buds regeneration. Similar results were obtained when regenerated buds of three different biomass dosages were cultured. It could be concluded from the above experimental results that for achieving better DA-6 application results, the concentration of DA-6 should be determined not only by the types but also by the biomass dosage of the explants. The present finding might help to improve the micropropagation efficiency in E. purpurea, and might be applicable for other species

KEYWORDS

Plant Growth Regulator, DA-6, Micropropagation, Biomass Dosage, Echinacea purpurea

Share and Cite:

Chen, X. , Li, D. , Zhang, J. , Li, Q. , Yang, Y. and Wu, H. (2016) The Biomass Dosage Influences the Effects of Diethyl Aminoethyl Hexanoate on Micropropagation of Echinacea purpurea (L.) Moench. Journal of Biosciences and Medicines, 4, 97-110. doi: 10.4236/jbm.2016.412014.

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