Author(s)

Tadashi Miura¹, Koji Tanabe^1,2, Eri Tsukagoshi², Koichi Kida^1,3, Yasuhiko Shizawa⁴, Nahoko Miyake⁵, Masataka Kasahara², Masao Yoshinari¹

¹Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Tokyo, Japan.
²Department of Pharmacology, Tokyo Dental College, Tokyo, Japan.
³Department of Oral and Maxillofacial Implantology, Tokyo Dental College, Tokyo, Japan.
⁴Department of Liberal Arts, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
⁵Department of Removable Partial Prosthodontics, Tokyo Dental College, Tokyo, Japan.

The aim of this study was to identify the potential antibacterial effects of gatifloxacin on periodontal pathogens including Aggregatibacter actinomycetemcomitans, Porphyromonas gingi-valis, and Prevotella intermedia. The minimum inhibitory concentrations (MIC) of gatifloxacin and its bactericidal effects were investigated. Gatifloxacin inhibited the growth of all three kinds of periodontopathic bacteria tested in broth. The MIC value of 2.5 nM was found to be the most effective in inhibiting A. actinomycetemcomitans. An adenosine triphosphate biolumi-nescence assay revealed that gatifloxacin exhibited bactericidal effects on the tested bacteria in a time-dependent manner. The safety of gatifloxacin in mammalian cells was evaluated by assessing the viability of normal human dermal fibroblast (NHDF) cells treated with gatifloxacin. Almost all NHDF cells survived after 2-d culture, while 81% of the cells survived after 4-d culture when treated with 1.0 × 10³ nM gatifloxacin. These results indicate that gatifloxacin is a possible drug for local administration to prevent periodontal infection.

Local Administration, Peri-Implantitis, Periodontopathic Bacteria, Antimicrobial Activity, Gatifloxacin

Miura, T. , Tanabe, K. , Tsukagoshi, E. , Kida, K. , Shizawa, Y. , Miyake, N. , Kasahara, M. and Yoshinari, M. (2016) Potential Antimicrobial Effects of Gatifloxacin on Periodontopathic Bacteria in Vitro. Journal of Biomedical Science and Engineering, 9, 354-359. doi: 10.4236/jbise.2016.97030.