Fatigue has negative impacts on the general working population as well as on seafarers. In order to study seafarers’ fatigue, a questionnaire-base survey was conducted to gain information about potential risk factors for fatigue and construct indexes indicating fatigue. The study applies T-test to compare strata of seafarers to analyse work and sleep patterns in global seafaring. Qualitative analysis are also employed to explore the impacts of fatigue on seafarer’s occupational health and safety.