Author(s)

Frank Anayo Orji^1*, Ekaette Nduka Dike¹, Adekunle Kolawole Lawal¹, Abdulateef Omoniyi Sadiq², Yewande Suberu¹, Ariyo Caleb Famotemi¹, Agatha Isioma Ugbana¹, Folake Fashola¹, Blossom Ita¹, Samuel Olakiitan Olatope¹, Emoleila Ejiya Itoandoan¹, Abimbola Olajumoke Adefiranye¹, Gloria Nwakaegho Elemo³

¹Department of Biotechnology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria.
²Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria.
³Department of Food Technology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria.

The properties of extracellular cellulase obtained from Bacillus species (FIIRO Strain B223) on supplying cellulose from Brewers Spent Grain (BSG) were studied. Generally, a crude cellulase activity between 11,757 Units to 13,930 Units was observed for both sources of carbon (BSG and crystalline cellulose). The optimum pH and temperature of 5.0, and 30°C - 40°C for both sources of carbon was observed. Heavy metals such as copper, zinc, and iron inhibited the activities of B223 cellulase while the cellulase activities on alkaline earth metal (manganese) were moderate. The co-factor behaviour of manganese ion was also established. Local production of cellulase in Nigeria using local raw materials such as Brewers Spent Grain reduces enzyme cost; cost of finished products and increases gross domestic products.

Cellulase, Bacillus species, Crude Activities, Temperature, pH Tolerance, Brewers Spent Grains (BSG)

Orji, F. , Dike, E. , Lawal, A. , Sadiq, A. , Suberu, Y. , Famotemi, A. , Ugbana, A. , Fashola, F. , Ita, B. , Olatope, S. , Itoandoan, E. , Adefiranye, A. and Elemo, G. (2016) Properties of Bacillus species Cellulase Produced Using Cellulose from Brewers Spent Grain (BSG) as Substrate. Advances in Bioscience and Biotechnology, 7, 142-148. doi: 10.4236/abb.2016.73013.