Sclerotia Formation and Toxin Production in Large Sclerotial Aspergillus flavus Isolates from Kenya - Advances in Microbiology

AiM > Vol.6 No.1, January 2016

Advances in Microbiology

Volume 6, Issue 1 (January 2016)

ISSN Print: 2165-3402 ISSN Online: 2165-3410

Google-based Impact Factor: 1.18 Citations h5-index & Ranking

Sclerotia Formation and Toxin Production in Large Sclerotial Aspergillus flavus Isolates from Kenya ()

HTML XML

Download as PDF (Size: 410KB) PP. 47-56

DOI: 10.4236/aim.2016.61005 4,519 Downloads 6,101 Views Citations

Author(s)

S. A. Okoth^1*, B. Nyongesa¹, V. Joutsjoki², H. Korhonen², V. Ayugi¹, E. K. Kang’ethe³

Affiliation(s)

¹School of Biological Sciences, University of Nairobi, Nairobi, Kenya.
²Natural Resources Institute Finland, Jokioinen, Finland.
³Department of Public Health, Pharmacology and Toxicology, University of Nairobi, Nairobi, Kenya.

ABSTRACT

We studied the relationship between sclerotia formation and aflatoxin production by Aspergillusflavus strains isolated from maize kernels from Nandi County. Isolates recovered from maize kernels were tested for their ability to form sclerotia on different growth media. PCR analysis was done on the isolates to detect 2 structural genes, aflD and aflQ, involved in aflatoxin biosynthesis pathway. Positive A. flavus isolates for one or both genes were grown on Yeast Extract Sucrose Agar medium and aflatoxins quantified using LCMSMS. All the isolates formed large sclerotia and their formation was influenced by media type but could not be related to amount of aflatoxins produced both in vivo and in vitro. Though sclerotia are perennating structures and so contribute to survival index of a fungus, their initiation is regulated by external factors though ability to form is genetic. This brings ambiguity of their presence or abundance as a measure of toxicity.

KEYWORDS

Sclerotia, Aflatoxins, Maize, Kenya, Aspergillus flavus

Share and Cite:

Okoth, S. , Nyongesa, B. , Joutsjoki, V. , Korhonen, H. , Ayugi, V. and Kang’ethe, E. (2016) Sclerotia Formation and Toxin Production in Large Sclerotial Aspergillus flavus Isolates from Kenya. Advances in Microbiology, 6, 47-56. doi: 10.4236/aim.2016.61005.

Cited by

[1]	Fungal Contamination and Characterization of Aspergillus flavus on Poultry Feeds and their Ingredients in North Sumatera
	CURRENT APPLIED …, 2023

[2]	Comparative Analysis of Multiple GWAS Results Identifies Metabolic Pathways Associated with Resistance to A. flavus Infection and Aflatoxin Accumulation in Maize
	Toxins, 2022

[3]	Occurrence of fungal contamination in ginseng sprout and mycotoxigenic potential
	Journal of Food …, 2021

[4]	Aspergillus Section Flavi from Four Agricultural Products and Association of Mycotoxin and Sclerotia Production with Isolation Source
	Current Microbiology, 2021

[5]	새싹삼의 곰팡이 발생과 독소생성능
	Jouranl of Food …, 2021

[6]	ОЦЕНКА ПОТЕНЦИАЛЬНОЙ ПАТОГЕННОСТИ ШТАММОВ ASPERGILLUS FLAVUS, ВЫДЕЛЕННЫХ ИЗ ОЧАГОВ БИОПОВРЕЖДЕНИЙ
	… : фундаментальные и прикладные …, 2021

[7]	Management strategies for aflatoxin risk mitigation in maize, dairy feeds and milk value chains—case study Kenya
	2021

[8]	Morphological Characterization and Determination of Aflatoxigenic and Non-Aflatoxigenic Aspergillus flavus Isolated from Sweet Corn Kernels and Soil in Malaysia
	2020

[9]	Management of Indoor Airborne Aspergillus flavus by Traditional Air Purifiers Commonly used in India
	2020

[10]	Scaling-Up the Impact of Aflatoxin Research in Africa. The Role of Social Sciences
	Toxins, 2018

[11]	Metabolites Identified during Varied Doses of Aspergillus Species in Zea mays Grains, and Their Correlation with Aflatoxin Levels
	Toxins, 2018

[12]	Polyphasic approach to the identification and characterization of aflatoxigenic strains of Aspergillus section Flavi isolated from peanuts and peanut-based products …
	International Journal of Food Microbiology, 2018

[13]	Fungal Population and Aflatoxin Contamination on Stored Gamma-Irradiated Nutmeg (Myristica fragrans) Kernels
	2018

[14]	SCREENING MAIZE INBRED LINES AND CROSSES FOR RESISTANCE TO AFLATOXIN AND FUMONISIN ACCUMULATION
	2018

[15]	Factors influencing aflatoxin contamination in maize at harvest and during storage in Kongwa district, Tanzania
	2017

[16]	Aspergillus flavus growth inhibition by Lactobacillus strains isolated from traditional fermented Kenyan milk and maize products.
	Archives of Microbiology, 2017

[17]	Aspergillus flavus growth inhibition by Lactobacillus strains isolated from traditional fermented Kenyan milk and maize products
	Archives of Microbiology, 2016

[18]	Capacidad de producción de aflatoxinas de aislamientos de Aspergillus provenientes del cultivo de frijol y su relación con el tamaño de los esclerocios
	2016

Journals Menu

Follow SCIRP

	+1 323-425-8868
	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies