Author(s)

Mohammad E. Khosroshahi^1,2*, Lida Ghazanfari^2,3, Payam Khoshkenar⁴

¹Center for Advanced Diffusion-Wave Technologies (CADIFT), Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Canada.
²Laser and Nanobiophotonics Laboratory, Biomaterial Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
³Max Planck Institute for the Science of Light, Erlangen, Germany.
⁴Department of Biomedical Engineering, College of Engineering & Science, Louisiana Technology University, Ruston, USA.

This paper investigated the numerical scheme extended to solve the hyperbolic non-Fourier form of bioheat transfer equation and the experimental trials were conducted to validate the numerical simulation. MNPs were prepared via co-precipitation and modified with a silica layer. The amino modified Fe₃O₄/SiO₂ nanoshells were covered with gold colloids producing nanoshells of Fe₃O₄/SiO₂/Au (MNSs). In vitro assays were performed to determine the effect of apoptosis of QU-DB lung cancer cells based on the cells morphology changes. Cell damage was reduced by decreasing the power density of laser. Also, a larger area of damage on cell culture plates was observed at longer intervals of laser irradiation. The effect of nanoshell concentration and irradiation rate has been evaluated. A maximum temperature rise of 6°C was achieved at 184 W/cm² and concentration of 0.01 mg/ml. The experiment confirmed a hyperbolic behaviour of thermal propagation. The results revealed that the three-dimensional implementation of bioheat equation is likely to be more accurate than the two-dimensional study.

Bioheat Equation, Gold Nanoshells, Laser Hyperthermia, Lung Cancer Cells, Surface Plasmon Resonance

Khosroshahi, M. , Ghazanfari, L. and Khoshkenar, P. (2014) Experimental Validation and Simulation of Fourier and Non-Fourier Heat Transfer Equation during Laser Nano-Phototherapy of Lung Cancer Cells: An in Vitro Assay. Journal of Modern Physics, 5, 2125-2141. doi: 10.4236/jmp.2014.518208.