Probing the Cell Cycle with Flow Cytometry - Journal of Biomedical Science and Engineering

JBiSE > Vol.7 No.9, July 2014

Journal of Biomedical Science and Engineering

Volume 7, Issue 9 (July 2014)

ISSN Print: 1937-6871 ISSN Online: 1937-688X

Google-based Impact Factor: 0.66 Citations h5-index & Ranking

Probing the Cell Cycle with Flow Cytometry ()

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DOI: 10.4236/jbise.2014.79069 5,421 Downloads 7,573 Views Citations

Author(s)

George D. Wilson

Affiliation(s)

Department of Radiation Oncology, Beaumont Health System, Royal Oak, MI, USA.

ABSTRACT

Flow cytometry is a versatile technique to study different aspects of the cell cycle from subpopulations of cells to detailed cell kinetic information. In this paper a basic review of cell kinetic parameters is presented followed by detailed descriptions of the different flow cytometric methodologies that can be used to extract pertinent information for a particular study. The methodologies range from simple DNA profile analysis, the use of bromodeoxyuridine to cell cycle-associated proteins such as the cyclins.

KEYWORDS

Flow Cytometry, Cell Cycle, DNA Profile, Bromodeoxyuridine, Cyclins

Share and Cite:

Wilson, G. (2014) Probing the Cell Cycle with Flow Cytometry. Journal of Biomedical Science and Engineering, 7, 698-711. doi: 10.4236/jbise.2014.79069.

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