Advances in Microbiology
Volume 4, Issue 7 (July 2014)
ISSN Print: 2165-3402 ISSN Online: 2165-3410
Google-based Impact Factor: 1.18 Citations h5-index & Ranking
Molecular Identification of Shiga-Toxin Producing and Enteropathogenic Escherichia coli (STEC and EPEC) in Diarrheic and Healthy Young Alpacas ()
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ABSTRACT
Isolation and biochemical and molecular identification of 303 strains of Escherichia coli obtained from diarrheic and healthy young alpacas of Puno-Peru, were realized. PCR amplification for 7 virulence factor genes associated with STEC, STEC O157:H7, EPEC: sxt1, sxt2, rfbO157, fliCH7, hlyA, eae y bfp were determined. A total of 39 strains (12.88%) showed amplification for one or more of these genes. Twenty three strains (59%) were classified as STEC and 16 strains (41%) as EPEC. An 88.18% (34/39) of STEC and EPEC strains were obtained from healthy alpacas and only 11.82% (5/39) from diarrheic alpacas considering this specie as potential zoonotic reservoir of STEC and EPEC.
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