The beneficial
effects of exercise have been recognized for many years yet the molecular mechanisms
by which exercise show benefits on health are still elusive. A combination of experimental
and bioinformatics approaches can be an invaluable tool to increase the
cellular understanding of the molecular mechanisms that underlie the aerobic
exercise in obese adolescents. Muscle skeletal proteins were separated by
2D-PAGE and changes in protein expression were revealed by ImageMaster 2D
Platinum analysis software. Proteins with expression changes after aerobic
exercise were identified by comparison with 2D maps from SWISS-2DPAGE and
interactions of proteins were analyzed with STRING and DAVID databases. After
aerobic exercise, all participants decreased glucose, insulin, total
cholesterol, body mass index and waist circumference. Proteomic differential
analysis revealed 10 overexpressed proteins after aerobic training, which
correspond to carbonic anhydrase III, beta enolase, creatine kinase, ATP
synthase beta subunit, aldolase A, glyceraldehyde-3-phosphate dehydrogenase,
triosephosphate isomerase, pyruvate kinase, lactate dehydrogenase and adenylate
kinase. Exercise increased glycolysis and oxidative phosphorylation pathways,
which is likely to be regulated by ubiquitin c protein. In conclusion, our
analysis suggested that obese adolescents show changes in their body mass
index, waist circumference and serum glucose after aerobic exercise through
improved protein expression patterns that aid, especially, glucose metabolism
and increase the oxidative phosphorylation. The most likely explanation for the
observed pattern is that ubiquitin c protein keeps the glycolytic enzymes bound
within skeletal muscle, obtaining a non-degradative role in cell signaling.