Author(s)

Julia Medrano Macías, Rahim Foroughbakhch Pournavab, Manuel Humberto Reyes-Valdés, Adalberto Benavides-Mendoza

Departamento de Fitomejoramiento, Universidad Autónoma Agraria Antonio Narro, Saltillo, México.
Departamento de Horticultura, Universidad Autónoma Agraria Antonio Narro, Saltillo, México.
Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, México.

A new, rapid and efficient reverse phase Liquid Chromatography (RP-LC) method was developed for determination of Gibberellin A4 (GA4) in samples of flower stalk of Dasylirion cedrosanum and vegetative tissue of Epithelantha micromeris. Purification of GA4 was carried out by solid phase extraction (SPE), in Epithelantha micromeris. In the chromatography method was obtaining a retention time of 2.1 min, using Hypersil GOLD C-18 column (100 × 4.6 mm dim and size particle 5 μ), mobile phase 50/50 acetonitrile/water and a flow 1.0 ml/min. Detection was carried out by a UV detector set at 205 nm, and a quantization limit of 0.4 mg/L. The obtained correlation coefficient was 0.995.

Dasyrilon cedrosanum; Epithelantha micromeris; Phytohormone Separation; Plant Growth Regulators; Reverse Phase Liquid Chromatography; Solid Phase Extraction

Macías, J. , Pournavab, R. , Reyes-Valdés, M. and Benavides-Mendoza, A. (2014) Development of a Rapid and Efficient Liquid Chromatography Method for Determination of Gibberellin A4 in Plant Tissue, with Solid Phase Extraction for Purification and Quantification. American Journal of Plant Sciences, 5, 573-583. doi: 10.4236/ajps.2014.55072.