Advances in Bioscience and Biotechnology
Volume 4, Issue 12 (December 2013)
ISSN Print: 2156-8456 ISSN Online: 2156-8502
Google-based Impact Factor: 1.18 Citations h5-index & Ranking
Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells ()
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ABSTRACT
Three transfection reagents, Lipofectamine® 2000, TransIT-PRO® and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced green fluorescent protein in Chinese hamster ovary cells. TransIT-PRO® was found to be more efficient under the examined conditions, but comes at an increased cost compared to the widely used PEI.
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