Advances in Biological Chemistry

Volume 3, Issue 6 (December 2013)

ISSN Print: 2162-2183   ISSN Online: 2162-2191

Google-based Impact Factor: 0.5  Citations  

Isolation and molecular characterization of a novel pseudomonas putida strain capable of degrading organophosphate and aromatic compounds

HTML  Download Download as PDF (Size: 2026KB)  PP. 564-578  
DOI: 10.4236/abc.2013.36065    5,133 Downloads   9,213 Views  Citations

ABSTRACT

A bacterial strain designated in this study as POXN01 was found to be capable of degrading the synthetic organophosphorus pesticides paraoxon and methyl parathion. The strain was initially isolated through enrichment technique from rice field soil near Harlingen, Texas. Phylogenetic analysis based on 16S rRNA, gyrB and rpoD gene alignments identified the POXN01 isolate as a new strain of Pseudomonas putida, which is closely related to the recently discovered nicotine-degrading strain Pseudomonas putida S16. While being unable to metabolize nicotine, the POXN01 isolate was observed to actively proliferate using monocyclic aromatic hydrocarbons, in particular toluene, as nutrients. Search for the genetic determinants of paraoxon catabolism revealed the presence of organophosphorus-degrading gene, opd, identical to the one from Sphingobium fuliginis (former Flavobacterium sp. ATCC 27551). Assimilation of aromatic compounds likely relies on phc ARKLMNOPQ gene cluster for phenol, benzene and toluene catabolism, and on benRABCDKGEF cluster for benzoate catabolism. The observed versatility of POXN01 strain in degradation of xenobiotics makes it useful for the multi-purpose bioremediation of contaminated sites in both agricultural and industrial environmental settings.

 

Share and Cite:

Iyer, R. , Stepanov, V. and Iken, B. (2013) Isolation and molecular characterization of a novel pseudomonas putida strain capable of degrading organophosphate and aromatic compounds. Advances in Biological Chemistry, 3, 564-578. doi: 10.4236/abc.2013.36065.

Cited by

[1] A novel organophosphate hydrolase from Arthrobacter sp. HM01: Characterization and applications
Bioresource …, 2022
[2] Degradation insight of organophosphate pesticide chlorpyrifos through novel intermediate 2, 6-dihydroxypyridine by Arthrobacter sp. HM01
Bioresources …, 2022
[3] Characteristics of miRNAs Present in Bovine Sperm and Associations With Differences in Fertility
Frontiers in …, 2022
[4] Operasi dan Remediasi Lingkungan
2022
[5] Biodegradation of Chlorpyrifos by Some Bacterial Strains and Screening Their Degraded Genes
Journal of Agricultural Chemistry …, 2022
[6] In-depth biochemical identification of a novel methyl parathion hydrolase from Azohydromonas australica and its high effectiveness in the degradation of various …
2021
[7] Biodegradable properties of organophosphorus insecticides by the potential probiotic Lactobacillus plantarum WCP931 with a degrading gene (opdC)
Applied …, 2021
[8] Performance Analysis of biosorption of Heavy Metal and Biodegradation PAH of Isolates Marine Sponges Symbiont Bacteria
Jurnal Akta Kimia …, 2021
[9] Eksplorasi spons indonesia: seputar kepulauan spermonde
2021
[10] Isolation, characterization and identification of organophosphate pesticide degrading bacterial isolates and optimization of their potential to degrade chlorpyrifos
2020
[11] Biodegradation of organophosphorus insecticides by two organophosphorus hydrolase genes (opdA and opdE) from isolated Leuconostoc mesenteroides WCP307 …
2020
[12] Microbial Metabolism of Organophosphates: Key for Developing Smart Bioremediation Process of Next Generation
2020
[13] Isolation, Characterization and Identification of Organophosphate Pesticide Degrading Bacterial Isolates and Optimization of their Potential to Degrade …
2020
[14] Protein-Based Bioscavengers of Organophosphorus Nerve Agents
2019
[15] Catalytic bioscavengers as countermeasures against organophosphate nerve agents
Chemico-Biological Interactions, 2018
[16] Biodegradation of Paraoxan as an Organophosphate Pesticide with Pseudomonas plecoglocissida Transfected by opd Gene
2017
[17] Biodegradation of Endosulfan as an Organochlorine Pesticide with Pseudomonas plecoglocissida Transfected by LinA Gene
2017
[18] Degradation of chlorpyrifos by an endophytic bacterium of the Sphingomonas genus (strain HJY) isolated from Chinese chives (Allium tuberosum)
Journal of Psychoactive Drugs, 2017
[19] Enhanced biodegradation of mixed PAHs by mutated naphthalene 1, 2-dioxygenase encoded by Pseudomonas putida strain KD6 isolated from petroleum refinery …
3 Biotech, 2017
[20] Environmentally effective photocatalyst CoO–TiO2 synthesized by thermal precipitation of Co in amorphous TiO2
Applied Catalysis B: Environmental, 2016
[21] Specific Gene Loci of Clinical Pseudomonas putida Isolates
PloS one, 2016
[22] Biodegradation and Bioremediation of Organic Chemical Pollutants by Pseudomonas
Pseudomonas: Molecular and Applied Biology, 2016
[23] Detection and location of OP-degrading activity: A model to integrate education and research
New biotechnology, 2015
[24] Environmentally effective photocatalyst CoO-TiO 2 synthesized by thermal precipitation of Co in amorphous TiO 2
Applied Catalysis B: Environmental, 2015

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.