Author(s)

Paul V. Flanagan, Brian P. Kelleher, Shane S. O’Reilly, Michal T. Szpak, Xavier Monteys, Paul P. Kelly, Anna N. Kulakova, Leonid A. Kulakov, Christopher C. R. Allen

Geological Survey of Ireland, Beggar’s Bush, Dublin, Ireland.
School of Biological Sciences, Queen’s University Belfast, Belfast, UK.
School of Chemical Sciences, Dublin City University, Dublin, Ireland.

A marine sediment core obtained from a methane seepage site off the northern coast of Irelandwas analysed, at 3 depths, for catabolic genes associated with the aerobic and anaerobic degradation of aromatic compounds. Catabolic gene copy numbers varied through the core peaking at 2.1 meters below sediment surface (mbsf)-just above the sulphate boundary. Beyond the sulphate boundary gene copy numbers fell considerably, suggesting the boundary may be a critical factor in the degradation of aromatic compounds within marine sediments. At the 2.1 mbsf depth our data also suggest that known benzoyl CoA reductase utilizing bacteria are readily detectable.

Aromatic Compounds; qPCR; bcr; bdo

P. Flanagan, B. Kelleher, S. O’Reilly, M. Szpak, X. Monteys, P. Kelly, A. Kulakova, L. Kulakov and C. Allen, "A Depth Resolved Insight into Benzoyl CoA Reductase and Benzoate Dioxygenase Gene Copy Numbers within a Marine Sediment Associated with Methane Seepage," Open Journal of Marine Science, Vol. 3 No. 4, 2013, pp. 175-181. doi: 10.4236/ojms.2013.34020.