Modern Instrumentation

Volume 2, Issue 3 (July 2013)

ISSN Print: 2165-9257   ISSN Online: 2165-9273

Google-based Impact Factor: 0.75  Citations  

A Low Cost and Versatile STED Superresolution Fluorescent Microscope

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DOI: 10.4236/mi.2013.23007    5,000 Downloads   10,277 Views  Citations

ABSTRACT

A versatile and inexpensive super-resolution fluorescent microscope that functions as easily as a conventional confocal microscope is described. Components of the microscope were designed on a platform which was placed atop a surplus microscope frame. All optical components and equipments used are given. The excitation and depletion beams are extracted from a compact low-cost supercontinuum light source. The focal spot of the depletion beam at the focal plane is studied and imaged by a 100 nm aperture near-field fiber tip. The collinear excitation and depletion beam focused by a 0.9 numerical aperture microscope objective produce a 90 nm lateral super-resolution as verified by imaging 100 nm diameter fluorescent beads.

Share and Cite:

D. McBride, C. Su, J. Kameoka and S. Vitha, "A Low Cost and Versatile STED Superresolution Fluorescent Microscope," Modern Instrumentation, Vol. 2 No. 3, 2013, pp. 41-48. doi: 10.4236/mi.2013.23007.

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