Modern Instrumentation
Volume 2, Issue 3 (July 2013)
ISSN Print: 2165-9257 ISSN Online: 2165-9273
Google-based Impact Factor: 0.75 Citations
A Low Cost and Versatile STED Superresolution Fluorescent Microscope ()
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ABSTRACT
A versatile and inexpensive super-resolution fluorescent microscope that functions as easily as a conventional confocal microscope is described. Components of the microscope were designed on a platform which was placed atop a surplus microscope frame. All optical components and equipments used are given. The excitation and depletion beams are extracted from a compact low-cost supercontinuum light source. The focal spot of the depletion beam at the focal plane is studied and imaged by a 100 nm aperture near-field fiber tip. The collinear excitation and depletion beam focused by a 0.9 numerical aperture microscope objective produce a 90 nm lateral super-resolution as verified by imaging 100 nm diameter fluorescent beads.
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