Author(s)

Malgorzata Madej, Claus Hansen, Anders Johannisson, Andrzej Madej

Danish Agriculture and Food Council, Copenhagen, Denmark.
Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.

The objectives of this study were to explore whether heparin-binding proteins, separated by fast protein liquid chromatography from boar seminal plasma influence the release of prostaglandins F_2α, (PGF_2α), E₂ (PGE₂) and interleukin-6 (IL-6) by porcine endometrial and cervical cells and even bovine endometrial cells. In Experiment I, we showed that release of PGF_2α by endometrial epithelial, endometrial stromal and cervical stromal cells to the medium was inhibited (p < 0.05) to 9.0% - 60.6% after 24 h incubation with 125 μg of heparin-binding proteins. Tumor necrosis factor α (TNFα) stimulated release of IL-6 by endometrial and cervical stromal cells after 24 h incubation, but in the presence of heparin-binding proteins, this stimulation was attenuated. Release of PGF_2α by cryopreserved (Experiment II) and primary (Experiment III) cervical stromal cells was significantly inhibited after 3 h incubation with 66 - 95.4 μg of heparin- binding proteins. A significant inhibition of PGE₂ release by cryopreserved and primary cervical stromal cells was already achieved after incubation with 16.5 - 23.9 μg of heparin-binding proteins. The release of IL-6 by cryopreserved cells was stimulated after 3 h incubation with heparin- binding proteins in a dose dependent manner in contrast to the release of IL-6 by freshly isolated cervical stromal cells. We also found (Experiment IV) that porcine heparin-binding seminal plasma proteins inhibited release of PGF_2α and stimulated release of IL-6 by bovine endometrial epithelial cells. In conclusion, a group of heparin-binding proteins separated by fast protein liquid chromatography from boar seminal plasma inhibit PGF_2α, PGE₂ and stimulate IL-6 release by porcine endometrial and cervical cells and even by bovine endometrial cells. Thus, these proteins have a similar effect as the entire seminal plasma.

Seminal Plasma Proteins; Cell Culture; Porcine; Bovine; Prostaglandins, Interleukin-6

Madej, M. , Hansen, C. , Johannisson, A. and Madej, A. (2013) Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells. Natural Science, 5, 21-30. doi: 10.4236/ns.2013.57A004.