Journal of Biomaterials and Nanobiotechnology

Volume 4, Issue 2 (April 2013)

ISSN Print: 2158-7027   ISSN Online: 2158-7043

Google-based Impact Factor: 1.69  Citations  

Study of Human Serum Albumin Adsorption and Conformational Change on DLC and Silicon Doped DLC Using XPS and FTIR Spectroscopy

HTML  Download Download as PDF (Size: 330KB)  PP. 194-203  
DOI: 10.4236/jbnb.2013.42024    8,490 Downloads   12,694 Views  Citations

ABSTRACT

Diamond-like carbon (DLC) coatings are extremely useful for creating biocompatible surfaces on medical implants. DLC and silicon doped DLC synthesised on silicon wafer substrate by using plasma enhanced chemical vapour deposition (PECVD). The effects of surface morphology on the interaction of HSA with doped and undoped DLC films have been investigated. The chemical composition of the surface before and after adsorption was analysed using X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR). Results showed that silicon incorporation DLC tends to increase of sp3/sp2 hybridization ratio by decreasing sp2 hybridized carbon bonding configurations. Following exposure to solutions containing (0.250 μg/ml) HSA, the results indicated that significant changes in the C, N and O levels on the surfaces with reducing of the Si2p band at 100 eV. From FTIR spectrum, the peaks occur the following functional groups were assigned as amide I and II groups at 1650 cm-1 and 1580 cm-1. Both XPS and FTIR spectroscopy confirm that HSA was bound onto the surfaces of the DLC and Si-DLC films via interaction of ionized carboxyl groups and the amino group did not play a significant role in the adsorption of protein. These results from peak intensity show that an adsorbed layer of HSA is higher at high level (19%) silicon doping. Therefore doping of DLC may provide an approach to controlling the protein adsorption.

Share and Cite:

M. Ahmed, J. Byrne, J. McLaughlin and W. Ahmed, "Study of Human Serum Albumin Adsorption and Conformational Change on DLC and Silicon Doped DLC Using XPS and FTIR Spectroscopy," Journal of Biomaterials and Nanobiotechnology, Vol. 4 No. 2, 2013, pp. 194-203. doi: 10.4236/jbnb.2013.42024.

Cited by

[1] Synthesis and catalytic properties of calcium oxide obtained from organic ash over a titanium nanocatalyst for biodiesel production from dairy scum
Chemosphere, 2022
[2] Simultaneous removal of heavy metals and bioelectricity generation in microbial fuel cell coupled with constructed wetland: an optimization study on substrate and …
Environmental Science and …, 2022
[3] Influence of carbon deposits on Fe-carbide for the Fischer-Tropsch reaction
Journal of …, 2022
[4] DLC coating over pre-oxidized and textured Ti6Al4V for superior adhesion and tribo-performance of hip implant
Surface and Coatings Technology, 2022
[5] Biomolecular corona formation on CuO nanoparticles in plant xylem fluid
2021
[6] Programmed BMP-2 release from biphasic calcium phosphates for optimal bone regeneration
2021
[7] Unveiling bonding states and roles of edges in nitrogen-doped graphene nanoribbon by X-ray photoelectron spectroscopy
Carbon, 2021
[8] A critical green biosynthesis of novel CuO/C porous nanocomposite via the aqueous leaf extract of Ficus religiosa and their antimicrobial, antioxidant, and …
Chemical Engineering Journal Advances, 2021
[9] Adsorption studies of Methylene Blue, Coomassie Brilliant Blue, and Congo Red dyes onto CuO/C nanocomposites synthesized via Vitex negundo Linn leaf …
Current Research in Green and Sustainable …, 2021
[10] A Unique Synthesis of Macroporous N-Doped Carbon Composite Catalyst for Oxygen Reduction Reaction
2021
[11] Simultaneous Removal of Heavy Metals And Bioelectricity Generation In Microbial Fuel Cell Coupled With Constructed Wetland: An Optimization Study On …
2021
[12] Microstructures, mechanical properties and tribological behaviors of amorphous carbon coatings in-situ grown on polycarbonate surfaces
2021
[13] The conversion reaction mechanism of bimetallic Ni–Fe hydroxycarbonate and its encapsulation in carbon nanospheres for achieving excellent Li-ion storage …
2020
[14] A Unique Synthesis of Macroporous N-Doped Carbon Composite Catalyst for Oxygen Reduction Reaction. Nanomaterials 2021, 11, 43
2020
[15] The fabrication of a new modified pencil graphite electrode for the electrocatalytic reduction of 2-nitrophenol in water samples
2020
[16] Green synthesis of Copper Oxide/Carbon nanocomposites using the leaf extract of Adhatoda vasica Nees, their characterization and antimicrobial activity
2020
[17] Effect of monovalent ions on the thermal stability of bovine serum albumin in dimethylsulfoxide aqueous solutions. Spectroscopic approach
2020
[18] Novel low cost proton exchange membrane made from sulphonated biochar for application in microbial fuel cells
2020
[19] Docetaxel loaded human serum albumin nanoparticles; synthesis, characterization, and potential of nuclear imaging of prostate cancer
2020
[20] Neutron refectometry analysis of nitrogen-containing DLC film structure for cell compatibility
JSAP Annual Meetings …, 2020
[21] 电极间距对 CW-MFC 处理污泥中 Zn 和 Ni 的效果及其产电性能的影响
化工进展, 2020
[22] Influence of RF power and CH4 flow rate on properties of diamond-like carbon films deposited by PECVD technique
2020
[23] Nanoparticles as carriers for the delivery of bevacizumab
2019
[24] Investigation of selective chemisorption of fcc and hcp Ru nanoparticles using X-ray photoelectron spectroscopy analysis
2019
[25] TEMPO-Functionalized Silica as an Efficient and Recyclable Oxidation Catalyst for Conversion of a Lignin Model Compound to Value-Added Products
2019
[26] Poly(γ-benzyl l-glutamate), by near-ambient pressure XPS
2019
[27] Сравнение методик расчета вторичной структуры белков на основе деконволюции инфракрасных спектров
2019
[28] 5.1. 5.1 Statement of Attribution
2019
[29] Pristine‐Graphene‐Supported Nitrogen‐Doped Carbon Self‐Assembled from Glucaminium‐Based Ionic Liquids as Metal‐Free Catalyst for Oxygen Evolution
2019
[30] Surface-modified PVdF-derived hierarchical mesoporous carbon matrix for high sulfur loading cathode in lithium–sulfur batteries
2019
[31] Si Doping Effects on the Growth of Large Single-crystal Diamond in Ni-based Metal Catalyst System under HPHT
2019
[32] Surface Characteristics and Biological Evaluation of Si-DLC Coatings Fabricated Using Magnetron Sputtering Method on Ti6Al7Nb Substrate
2019
[33] Synchrotron-based NEXAFS analysis of thermal-treated diamond-like carbon films
2019
[34] Artificial intelligence-based behavioral analysis of protein in a nanoscale cubic space on a Si substrate
2019
[35] Comparison of protein secondary structure calculation methods based on infrared spectra deconvolution
2019
[36] Pristine Graphene Supported Nitrogen‐Doped Carbon in Situ Self‐Assembled from Glucaminium‐Based Ionic Liquids as a Superior Metal‐Free Catalyst toward …
2019
[37] Human serum albumin nanoparticles for ocular delivery of bevacizumab
International Journal of Pharmaceutics, 2018
[38] Effects on thermophysical properties of carbon based nanofluids: Experimental data, modelling using regression, ANFIS and ANN
International Journal of Heat and Mass Transfer, 2018
[39] Effects of ordered mesoporous bimodal structures of Fe/KIT-6 for CO hydrogenation activity to hydrocarbons
Chemical Engineering Journal, 2018
[40] Facile and effective antibacterial coatings on various oxide substrates
Journal of Industrial and Engineering Chemistry, 2018
[41] Structural, optoelectronic and mechanical properties of PECVD Si-CN films: An effect of substrate bias
Materials Science in Semiconductor Processing, 2018
[42] Development of waste biomass based sorbent for removal of cyanotoxin microcystin-LR from aqueous phases
Bioresource Technology, 2018
[43] The correlation between optical and mechanical properties of amorphous diamond-like carbon films prepared by pulsed filtered cathodic vacuum arc deposition
Thin Solid Films, 2018
[44] Raman spectroscopy study of the optical properties of human serum albumin with dye aqueous solution droplet in presence of silver nanoparticles
Proceedings Volume 10672, Nanophotonics VII, 2018
[45] Biomineralization of osteoblasts on DLC coated surfaces for bone implants
2018
[46] Combined IR and XPS characterization of organic refractory residues obtained by ion irradiation of simple icy mixtures
2018
[47] Artificial intelligence-based inspection of contact shock of a functional protein on a silicon substrate
2018
[48] Improvement in tribology and corrosion resistance of diamond-like carbon film by titanium doping using the filtered cathodic vacuum arc technique
2018
[49] Characterization and enhancement of IR optical and tribological properties of DLC films synthesized by RF-PECVD
2017
[50] ELECTROSYNTHESIS OF COPPER OXIDE SUPPORTED ON AMORPHOUS TITANIUM DIOXIDE NANOPARTICLES BASED CATALYSTS FOR …
Thesis, 2017
[51] Scale Resistant Production Tools
2017
[52] Preparation of Dumbbell-like Er/ZnO Microrods with Efficient Energy Upconversion for the Catalytic Degradation of Tartaric Acid in Water
Topics in Catalysis, 2017
[53] Highly Ordered Mesoporous Fe2O3–ZrO2 Bimetal Oxides for an Enhanced CO Hydrogenation Activity to Hydrocarbons with Their Structural Stability
ACS Catalysis, 2017
[54] One-step prepared Cobalt-based Nanosheet as an Efficient Heterogeneous Catalyst for Activating Peroxymonosulfate to Degrade Caffeine in Water
Journal of Colloid and Interface Science, 2017
[55] GalNAc bio-functionalization of nanoparticles assembled by electrostatic interactions improves siRNA targeting to the liver
Journal of Controlled Release, 2017
[56] Chelating functional group attached to carbon nanotubes prepared for performance enhancement of vanadium redox flow battery
Journal of Materials Chemistry A, 2017
[57] Surface engineering of Ti6Al4V surfaces for enhanced tribocorrosion performance in artificial seawater
Materials & Design, 2016
[58] Backfilling‐Free Strategy for Biopatterning on Intrinsically Dual‐Functionalized Poly [2‐Aminoethyl Methacrylate‐co‐Oligo (Ethylene Glycol) Methacrylate] Films
Chemistry–An Asian Journal, 2016
[59] Importance of the coating pH in fabrication of polyethylenimine-coated polysulfone-Escherichia coli composite fiber sorbent
Journal of the Taiwan Institute of Chemical Engineers, 2016
[60] Plasma-Assisted Fabrication and Processing of Biomaterials
2016
[61] Influence of the silicon and oxygen content on the properties of non-hydrogenated amorphous carbon coatings
Diamond and Related Materials, 2016
[62] Backfilling‐Free Strategy for Biopatterning on Intrinsically Dual‐Functionalized Poly[2‐Aminoethyl Methacrylate‐co‐Oligo(Ethylene Glycol) Methacrylate] Films
Chemistry–An Asian Journal, 2016
[63] Effect of ZDDP concentration on the thermal film formation on steel, hydrogenated non-doped and Si-doped DLC
Applied Surface Science, 2016
[64] Plasma‐Assisted Fabrication and Processing of Biomaterials
… of polymeric materials …, 2016
[65] Covalent Attachment of Anderson‐Type Polyoxometalates to Single‐Walled Carbon Nanotubes Gives Enhanced Performance Electrodes for Lithium Ion Batteries
Chemistry-A European Journal, 2015
[66] Fabrication of Multifunctional SiO2@GN-Serum Composites for Chemo-Photothermal Synergistic Therapy
ACS Applied Materials & Interfaces, 2015
[67] Surface functionalization of Bioglass? with Alkaline Phosphatase
Surface and Coatings Technology, 2015
[68] Characteristic of silicon doped diamond like carbon thin films on surface properties and human serum albumin adsorption
Diamond and Related Materials, 2015
[69] Kinetics and thermodynamics of human serum albumin adsorption on silicon doped diamond like carbon
Materials Chemistry and Physics, 2015
[70] Smart porous silicon nanoparticles with polymeric coatings for sequential combination therapy
Molecular pharmaceutics, 2015
[71] Functional gold nanoparticle coated surfaces for CA 125 cancer biomarker detection
2015
[72] Multiband photoluminescence from carbon nanoflakes synthesized by hot filament CVD: towards solid-state white light sources
Journal of Materials Chemistry C, 2014
[73] Adsorption of Human Serum Albumin (HSA) by SWNTs/Py-PW11 Nanocomposite
Industrial & Engineering Chemistry Research, 2014
[74] Effect of surface modification of poly (lactic acid) by low-pressure ammonia plasma on adsorption of human serum albumin
Applied Surface Science, 2014
[75] Fabrication of Multifunctional SiO2@ GN-Serum Composites for Chemo-Photothermal Synergistic Therapy
ACS applied materials & interfaces, 2014
[76] Insulin unfolding and aggregation: a multi-disciplinary study
Thesis, 2014
[77] Enzymatic activity induced by interactions with a nanofabricated hydrophobic Si surface
Applied Physics Letters, 2013
[78] 3-amino-1, 2, 4-triazol-5-tiyol ile modifiye edilen kalem grafit elektrot yüzeyinde 2-nitrofenolün elektrokimyasal davranışı ve tayini

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.