Author(s)

Nowruz Delirezh, Seyed Mohammad Moazzeni, Fazel Shokri, Mohammad Ali Shokrgozar, Morteza Morteza Atri, Hamid Karbassian

Cancer Institute, Imam Hospital, Tehran University of Medical Science, Tehran, Iran.
Department of Cellular and Molecular, Institute of Biotechnology, Urmia University, Urmia, Iran.
Department of Immunology, School of Health, Tehran University of Medical Science, Tehran, Iran.
Department of Immunology, School of Medical Science, Trabiat Modares University, Tehran, Iran.
Department of Surgery, Athieh Hospital, Tehran, Iran.
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.

In this In vitro study, T cell responses induced by breast tumor cell lysate pulsed monocyte-derived DCs were analyzed in terms of proliferation, specific cytotoxicity and cytokine-release in order to use in immunotherapeutic settings. Nylon wool enriched T lymphocytes from 5 patients with breast cancer stimulated In vitro with tumor cell lysate pulsed monocyte-derived DCs and their proliferation response were analyzed by [³H] thymidine uptake test. Specific cytotoxic activity of tumor antigen primed T cells after three rounds weekly stimulation was evaluated by flow cytometry, and interferon-γ (IFN-γ) and interleukin-4 (IL-4) cytokines release assay was carried out 24 hours after last stimulation in the supernatant of primed T cells using commercially available ELI-SA kits. T cell proliferation assay revealed that tumor cell lysate pulsed DCs could stimulate autologous T cell proliferation response with stimulation indices 4.9 - 30. T cell mediated cytotoxicity assay demonstrated that tumor antigen primed T cells could significantly kill autologous tumor cells more than normal cells (P < 0.05). These cells had variable amounts of cytotoxic activity against K562 cells as well. Primed T cells were released both IFN-γ and IL-4 in response to restimulation by antigen pulsed DCs which were dominated by IFN-γ production in 2 and IL-4 production in 3 out of 5 patients. Our result suggested that breast tumor antigen pulsed DCs could elicit effective specific antitumor T cell responses In vitro, therefore, tumor antigen pulsed DC vaccination may be considered as a novel strategy for immunotherapy of patients with breast cancer refractory to standard modalities.

Breast; Lysate; Dendritic Cells; T Cell

Delirezh, N. , Moazzeni, S. , Shokri, F. , Shokrgozar, M. , Morteza Atri, M. and Karbassian, H. (2012) In vitro analysis of T cell responses induced by breast tumor cell lysate pulsed with autologous dendritic cells. Advances in Bioscience and Biotechnology, 3, 126-136. doi: 10.4236/abb.2012.32019.