Determination of 8 Diuretics and Probenecid in Human Urine by Gas Chromatography-Mass Spectrometry: Confirmation Procedure - American Journal of Analytical Chemistry

AJAC > Vol.3 No.4, April 2012

American Journal of Analytical Chemistry

Volume 3, Issue 4 (April 2012)

ISSN Print: 2156-8251 ISSN Online: 2156-8278

Google-based Impact Factor: 1.71 Citations h5-index & Ranking

Determination of 8 Diuretics and Probenecid in Human Urine by Gas Chromatography-Mass Spectrometry: Confirmation Procedure ()

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DOI: 10.4236/ajac.2012.34044 5,607 Downloads 10,254 Views Citations

Author(s)

Olga Zaporozhets, Iuna Tsyrulneva, Mykola Ischenko

Affiliation(s)

Taras Shevchenko National University of Kiev, Kiev, Ukraine.

ABSTRACT

A fast and sensitive method for determination of 8 diuretics (acetazolamide, bendroflumethiazide, bumetanide, chlorthalidone, furosemide, hydrochlorothiazide, metolazone, triamterene) and masking agent (probenecid) in human urine using gas-chromatography with mass spectrometric detection is described. The extraction of the substances as function of the nature of organic solvent, mixing time and pH of aqueous phase was studied. The tandem mass spectrometry was used to increase selectivity of diuretics determination due to elimination of background interferences. Fragmentation reactions were studied for each compound and their collision energies were optimized to obtain the best selectivity. The results of method’s validation demonstrate its suitability in routine analysis for confirmation purposes.

KEYWORDS

Diuretics; Urine; Gas Chromatography; Tandem Mass Spectrometry; Confirmation

Share and Cite:

O. Zaporozhets, I. Tsyrulneva and M. Ischenko, "Determination of 8 Diuretics and Probenecid in Human Urine by Gas Chromatography-Mass Spectrometry: Confirmation Procedure," American Journal of Analytical Chemistry, Vol. 3 No. 4, 2012, pp. 320-327. doi: 10.4236/ajac.2012.34044.

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