Author(s)

Christian Ahadi Irenge^1*, Freddy Bikioli², Patient Birindwa Mulashe¹, Félicien Mushagalusa Kasali¹, Patient Wimba³, Aksanti Lwango⁴, Yves Coppieters⁵, Justin Ntokamunda Kadima^1,6

¹Department of Pharmacy and Public Health, Official University of Bukavu, Bukavu, Democratic Republic of the Congo.
²Department of Tropical Medecine, Faculty of Medecine, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
³Department of Microbiology, Faculty of Medicine, Official University of Bukavu, Bukavu, Democratic Republic of the Congo.
⁴Department of Medical Biology, Higher Institute of Medical Technics, Bukavu, Democratic Republic of the Congo.
⁵School of Public Health, Free University of Bruxelles, Bruxelles, Belgium.
⁶School of Medicine, College of Medicine and Health Sciences, University of Rwanda, Huye, Rwanda.

Objective: To evaluate the spread of Multidrug-Resistant (MDR) bacterial infections in Bukavu hospitals and test antimicrobial susceptibility patterns of some isolates to usual marketed antibiotics. Methods: The prevalence of MDR strains was determined by using general antimicrobial susceptibility data collected from 3 hospital laboratories. The susceptibility of some isolates to usual antibiotics was processed by agar diffusion method with standard E. coli ATCC8739 and standard antibiotics discs as controls. The tested antibiotics were ampicillin, ceftriaxone, gentamicin, chloramphenicol and ciprofloxacin. Results: At the 3 hospitals, 758 tests were realized in urine, pus, stool, FCV, blood, LCR, split and FU specimens; 46 strains were unidentified and 712 strains were identified. Of 712 identified strains, 223 (31.4%) were MDR or XDR strains including Escherichia coli, Klebsiella pneumoniae, Enterobacter, Proteus mirabilis, Salmonella enterica, Pseudomonas aeruginosa, Citrobacter freundii, Morganella morganii, Enterococcus faecalis and E. faecium, Neisseria gonorrohoae, Staphylococcus aureus, coagulase-negative, staphylococci, Streptococcus pneumoniae and Streptococcus pyogenes. Of the infected patients, 36 (21.5%) children were under 16 years and 188 (78.5%) adults were predominately women (58.5%). The susceptibility test showed that all strains but S. aureus were resistant to ampicillin and amoxicillin and ciprofloxacin. Gentamicin, ceftriaxone, and chloramphenicol remain partially active (27% - 80%) against P. mirabilis, E. coli and P. aeruginosa. The resistance is more likely related to strain mutation than to pharmaceutical quality of the antibiotics prescribed. Conclusion: Both data from hospital laboratories and in vitro post-testing findings confirmed the ongoing elevated prevalence of MDR strains in Bukavu. The causes of antibiotic misuse and socio-economic determinants of the phenomenon of resistance should be scrutinized in order to take adequate strategies in the prospective of establishing an effective control system against this threat to overall health. The results of this work on MDR profiles have various implications for the management of infectious diseases. It provides indicators for the surveillance of antimicrobial resistance, practical guidelines for antibiotic susceptibility testing in biomedical laboratories, and guidance for antibiotic therapy.

Prevalence, Antimicrobials, Multi-Resistance, Bacterial Sensitivity, Bukavu, DRC

Irenge, C. , Bikioli, F. , Mulashe, P. , Kasali, F. , Wimba, P. , Lwango, A. , Coppieters, Y. and Kadima, J. (2024) Profile of Multidrug Resistant Bacteria in Bukavu Hospitals and Antimicrobial Susceptibility to Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus. Advances in Microbiology, 14, 209-225. doi: 10.4236/aim.2024.144015.