Author(s)

Mariétou Konate^1*, Aminata Fofana^#2*, Yacouba Kouadima³, Aboubacar Sidiki Ouattara⁴, Adama Sanou⁵

¹UFR/Sciences et Techniques, Université Catholique de l’Afrique de l’Ouest, Unité Universitaire à Bobo (UCAO/UUB), Bobo-Dioulasso, Burkina Faso.
²Departement Biomédical et Santé Publique (BioMed/SP), Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso, Burkina Faso.
³Departement de Biochimie-Microbiologie, UFR Sciences de la Vie et de la Terre, Université Nazi BONI, Bobo-Dioulasso, Burkina Faso.
⁴Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso.
⁵Laboratoire de Mycobactériologie, Centre MURAZ, Institut National de Santé Publique, Bobo-Dioulasso, Burkina Faso.

Bovine tuberculosis (bTB) is an endemic zoonosis significantly affects animal health in Burkina Faso. The primary causative agent is Mycobacterium tuberculosis (M. tuberculosis) complex, mainly M. bovis. Cattle are considered as natural reservoir of M. bovis. However, in Burkina Faso, the circulation of these strains remains poorly understood and documented. This study aimed to identify and characterize Mycobacterium strains from suspected carcasses during routine meat inspection at Bobo-Dioulasso refrigerated slaughterhouse. A prospective cross-sectional study was conducted from January 2021 to December 2022 on cases of seizures linked to suspected bovine tuberculosis. Microbiological and molecular analyzes were used for mycobacterial strain isolation and characterization. Out of 50 samples, 24% tested positive by microscopy and 12% by culture. Molecular analysis identified 6 strains of Mycobacteria, exclusively Mycobacterium bovis specifically the subspecies bovis (Mycobacterium bovis subsp bovis). In conclusion, M. bovis subsp bovis is the primary agent responsible for bovine tuberculosis in Bobo-Dioulasso. Continuous monitoring of mycobacterial strains is therefore necessary for the effective control of this pathology in the local cattle population.

Bovine Tuberculosis, Mycobacterium bovis, Molecular Identification, Cattle Population, Burkina Faso

Konate, M. , Fofana^#, A. , Kouadima, Y. , Ouattara, A. and Sanou, A. (2024) Molecular Identification of Mycobacterium Strains Responsible of Bovine Tuberculosis Cases in Bobo-Dioulasso Slaughterhouse, Burkina Faso. Advances in Microbiology, 14, 105-114. doi: 10.4236/aim.2024.142008.