Author(s)

Mana Fuchigami^1*, Osamu Tsuzukibashi¹, Akira Fukatsu¹, Koji Umezawa², Sachiyo Hayashi², Yuji Takahashi³, Hiroshi Yamamoto⁴, Chiaki Komine¹, Mio Hagiwara-Hamano⁴, Yukiko Iizuka⁴, Satoshi Uchibori⁵, Masanobu Wakami⁵, Hiroshi Murakami³, Taira Kobayashi⁵, Masahiko Fukumoto¹

¹Department of Laboratory Medicine for Dentistry for the Compromised Patient, School of Dentistry at Matsudo, Nihon University, Chiba, Japan.
²Department of Special Needs Dentistry, School of Dentistry at Matsudo, Nihon University, Chiba, Japan.
³Department of Oral Implantology, School of Dentistry at Matsudo, Nihon University, Chiba, Japan.
⁴Department of Oral Surgery, School of Dentistry at Matsudo, Nihon University, Chiba, Japan.
⁵Department of Fixed Prosthodontics, School of Dentistry at Matsudo, Nihon University, Chiba, Japan.

Purpose: Recently, Candida haemulonii complex (Candida haemulonii, Candida duobushaemulonii and Candida haemulonii var. vulnera) and two genetically close species (Candida pseudohaemulonii and Candida auris) have emerged as an opportunistic fungal pathogen associated with various infectious diseases of humans, and most of those isolates have displayed antifungal resistance. Because it is difficult to differentiate these microorganisms by a current technique, unfortunately, it is important to establish a method for identifying them accurately. The purpose of the present study was to design species-specific primers in order to identify and detect C. auris, C. pseudohaemulonii, and C. haemulonii complex, i.e. , C. haemulonii, C. duobushaemulonii and C. haemulonii var. vulnera using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the 26S rRNA, 18S rRNA, and RPB1 genes and ITS region of five Candida species. Results: The multiplex PCR method developed in this study was able to distinguish five Candida species clearly. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction.

Genus Candida, Candida haemulonii complex, Multiplex PCR, Candida auris

Fuchigami, M. , Tsuzukibashi, O. , Fukatsu, A. , Umezawa, K. , Hayashi, S. , Takahashi, Y. , Yamamoto, H. , Komine, C. , Hagiwara-Hamano, M. , Iizuka, Y. , Uchibori, S. , Wakami, M. , Murakami, H. , Kobayashi, T. and Fukumoto, M. (2023) One Step Multiplex PCR for Identification of Candida haemulonii complex and Candia auris. Advances in Microbiology, 13, 602-613. doi: 10.4236/aim.2023.1312039.