Author(s)

Bawomon Fidèle Neya^1,2, Tobdem Gaston Dabire¹, Alassane Ouattara², Amado Sawadogo², Diariétou Sambakhe³, Kouka Hamidou Sogoba², Tounwendsida Abel Nana², Ibié Gilles Thio⁴, Frank Essem⁵, Kadidia Koita²

¹University Center of Gaoua, Nazi BONI University, Bobo Dioulasso, Burkina Faso.
²Life and Earth Sciences Unit, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.
³Senegalese Institute of Agricultural Research/CERAAS, Thiès, Senegal.
⁴Institute for the Environment and Agricultural Research (INERA), Ouagadougou, Burkina Faso.
⁵Akenten Appiah-Menka University of Skills Training and Entrepreneurial Development, Mampong, Ghana.

Late leaf spot caused by Cercosporidium personatum is one of the most widespread groundnut leaf diseases. Along with early leaf spot and rust, it is one of the main fungal diseases hampering groundnut production worldwide. Late leaf spot accounts for significant yield losses throughout the world where groundnuts are grown. This reduction in yield caused by the disease could result in yield losses of between 50% and 70%. In Burkina Faso, the disease is present throughout the country, with incidence varying according to region and season. Could the variability in the incidence of the disease be linked to the nature of the isolates or to the conditions from each agro-ecological zone? In this study, the aim was to assess the capacity of three isolates from three agroclimatic zones of the country to defoliate and reduce groundnut yield. To this end, three isolates of Cercosporidium personatum (Berk. Et M.A Curt.) were collected in these zones and evaluated on three contrasting groundnut varieties. A split-splot design was used for the experiment. Isolates were prepared from samples collected in farmers’ fields. After incubation in the laboratory, leaf spots showing good sporulation were scraped off with a scalpel after immersing the leaves in distilled water. Inoculations were carried out under controlled environment. After inoculation with the isolate, the percentage of defoliation and the reduction in yield of these varieties were then evaluated. Inoculation was carried out from the 30th day after sowing with Cercosporidium personatum spore suspensions at 10⁵ spores/ml. The study showed that the percentage of defoliation (P = 0.0001) and the reduction in yield (P = 0.0001) were significant. The study revealed that, whatever the variety, isolate I3TF from the Upper Basins region in the South Sudanese zone caused the greatest defoliation and the greatest reduction in yield. The variety TS32-1, regardless of the isolate used for the treatment, recorded the best yield. The variety PC79-79, regardless of the isolate used for the treatment, recorded the lowest percentage of defoliation. The highest defoliation recorded under the effect of the isolates was of the order of 72.20%; the highest yield reduction was of the order of 87.20% compared with the water control.

Arachis hypogaea, Isolate, Late Leaf Spot, Cercosporidum personatum, Yield, Defoliation

Neya, B. , Dabire, T. , Ouattara, A. , Sawadogo, A. , Sambakhe, D. , Sogoba, K. , Nana, T. , Thio, I. , Essem, F. and Koita, K. (2023) Impact of Three Isolates of Cercosporidium personatum (Berk. Et M.A Curt.), a Pathogen of Late Leaf Spot, on Defoliation and Yield in Groundnut (Arachis hypogaea L.) under Controlled Conditions. American Journal of Plant Sciences, 14, 1101-1114. doi: 10.4236/ajps.2023.1410075.