Author(s)

Lydia Tasi¹, Jayaprakash Reddy¹, Mohammad Yazid Abdad², Amenda Wilson¹

¹Department of Agriculture, Papua New Guinea University of Technology, Lae, Papua New Guinea.
²Papua New Guinea Institute of Medical Research, Goroka, Papua New Guinea.

This trial was conducted to identify the prevalence of Salmonella and Campylobacter in different farming practices: commercial, semi-commercial, and subsistence farms in the four regions; Southern, Highland, Momase, and New Guinea Island (NGI) in PNG. The research was conducted at Institute of Med-ical Research (IMR) at Goroka, Eastern Highland Province. The samples used in the experiment were retrospective samples that were collected in 2011 and 2012 among the four regions and at different farming sites. A total of 333DNA samples were extracted from cloacal swabs. These samples were subjected to molecular assay where DNA extraction was done and analysed using re-al-time polymerase chain reaction (PCR). From the result, it was evident that in Southern region, significant differences were observed only in commercial and semi-commercial farms (p-value = 0.0030) and semi-commercial and non-commercial farms (p-value = 0.0002). However, in NGI significant dif-ferences were not observed in all the farming practices (p-value < 0.05). Significant differences were observed between Campylobacter in commercial and non-commercial (p-value = 0.0006) and semi-commercial and non-commercial farm (p-value < 0.0001) in Momase region. The Highlands regions showed significant differences of Campylobacter spp. between commercial and semi-commercial (p-value < 0.0001) and commercial and non-commercial (p-value < 0.0001) farming practices.

Deoxyribo Nucleic Acid, Polymerase Chain Reaction, Salmonella, Campylobacter, Molecular Assay, Poultry

Tasi, L., Reddy, J., Abdad, M.Y. and Wilson, A. (2023) Prevalence of Salmonella and Campylobacter in Chicken among the Different Farming Systems in Papua New Guinea (PNG). Open Access Library Journal, 10, 1-11. doi: 10.4236/oalib.1107851.