Author(s)

Winnie Tatiana Bekolo Nga^1,2*, Guy Roger Nsenga Djapa³, Kelly Ilinga Meme Mamende¹, Antonin Wilson Ndjitoyap Ndam⁴, David Sepo Sepo⁵, Agnès Malongue², Firmin Ankouane Andoulo⁴, Oudou Njoya⁴, Henry Luma Namme¹, Elie Claude Ndjitoyap Ndam⁴, Carole Else Eboumbou¹, Servais Albert Fiacre Eloumou Bagnaka¹

¹Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon.
²Department of Medecine, Douala General Hospital, Douala, Cameroon.
³Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.
⁴Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon.
⁵Department of Internal Medecine, Douala Military Hospital, Douala, Cameroon.

Introduction: Helicobacter pylori is a gram-negative bacillus responsible for numerous gastroduodenal pathologies, and this infection is a public health problem. The prevalence of infection with this bacterium remains high in countries with limited resources. Diagnosis relies mainly on numerous invasive and noninvasive methods. The aim of this work was to evaluate the different indirect diagnostic methods using bacterial cultures. Methods: We conducted a cross-sectional and analytical study from January to May 2022 in the gastroenterology departments of Douala General Hospital and Douala Military Hospital. All patients aged 18 years and older who were in the gastroenterology consultation and agreed to participate were included in our study. Sociodemographic, clinical, and paraclinical data were collected. Urease, liquid urea, and culture tests were performed from the specimens obtained by fibroscopy. Serological tests were performed on the blood sample. Results: 101 patients were included, 58 were female and 43 were male, for a sex ratio of 1.3. The mean age was 44.2 ± 16 years. The prevalence of infection was 90.5%, 44.1%, 40.6% and 21.8% for serology, direct microbiological examination, RUT (rapid urea test) and culture, respectively. Comparison of the different tests showed sensitivity and specificity of 67.1% and 64%, respectively, for RUT, 100% and 73.7%, respectively, for direct microbiological examination, and 100% and 14.8%, respectively, for serology. The positive and negative predictive values were 39.5% and 100% for serology, 39% and 85% for RUT, and 55.6% and 100% for direct microbiological examination, respectively. Conclusion: The prevalence of Helicobacter pylori infection depends on the type of test used. Direct examination is more reliable than RUT and serology.

Helicobacter pylori, Diagnostic Tests, Sensitivity, Specificity, Positive and Negative Predictive Value

Nga, W. , Djapa, G. , Mamende, K. , Ndam, A. , Sepo, D. , Malongue, A. , Andoulo, F. , Njoya, O. , Namme, H. , Ndam, E. , Eboumbou, C. and Bagnaka, S. (2023) Diagnosis of Helicobacter pylori Infection in Low Out-Outcome Country: Rapid Urease Test, Serological Test, versus Direct Microbiological Examination with Gram Stain. Open Journal of Gastroenterology, 13, 199-208. doi: 10.4236/ojgas.2023.136019.