Author(s)

Elizabeth Cagle¹, Brent Lake¹, Anasua Banerjee¹, Jazmine Cuffee¹, Narendra Banerjee¹, Darla Gilmartin¹, Makaiyah Liverman¹, Shennel Brown¹, Erik Armstrong¹, Santanu Bhattacharya^2,3, Somiranjan Ghosh⁴, Tanmoy Mandal⁴, Hirendra Banerjee^1*

¹Department of Natural, Health and Human Sciences, Elizabeth City State University Campus of The University of North Carolina, Elizabeth, NC, USA.
²Department of Biochemistry and Molecular Biology, Mayo College of Medicine and Science, Jacksonville, FL, USA.
³Department of Physiology and Biomedical Engineering, Mayo College of Medicine and Science, Jacksonville, FL, USA.
⁴Departments of Pediatrics and Child Health, College of Medicine, Howard University, Washington, DC, USA.

Triple Negative Breast Cancer (TNBC) is a malignant form of cancer with very high mortality and morbidity. Epithelial to Mesenchymal Transition (EMT) is the most common pathophysiological change observed in cancer cells of epithelial origin that promotes metastasis, drug resistance and cancer stem cell formation. Since the information regarding differential gene expression in TNBC cells and cell signaling events leading to EMT is limited, this investigation was done by comparing transcriptomic data generated by RNA isolation and sequencing of a EMT model TNBC cell line in comparison to regular TNBC cells. RNA sequencing and Ingenuity Pathway Software Analysis (IPA) of the transcriptomic data revealed several upregulated and downregulated gene expressions along with novel core canonical pathways including Sirtuin signaling, Oxidative Phosphorylation and Mitochondrial dysfunction events involved in EMT changes of the TNBC cells.

Triple Negative Breast Cancer, Epithelial to Mesenchymal Transition, Core Canonical Pathways

Cagle, E. , Lake, B. , Banerjee, A. , Cuffee, J. , Banerjee, N. , Gilmartin, D. , Liverman, M. , Brown, S. , Armstrong, E. , Bhattacharya, S. , Ghosh, S. , Mandal, T. and Banerjee, H. (2023) Analysis of Differential Gene Expression and Core Canonical Pathways Involved in the Epithelial to Mesenchymal Transition of Triple Negative Breast Cancer Cells by Ingenuity Pathway Analysis. Computational Molecular Bioscience, 13, 21-34. doi: 10.4236/cmb.2023.132002.