Responses Taken by Silencing of NFkappaB, STAMP1 and STAMP2 Genes and Expression of NFkB, Act-1, p53 and p73 at -/+ TNFalpha Induced LNCaP Cells ()
ABSTRACT
Prostate cancer is the most commonly diagnosed
cancer and the second leading cause of
cancer mortality in men in the Western World. The effects of androgens are
mediated by the Androgen Receptor (AR). Therefore, studies focus on the identification of AR-regulated genes that are also highly expressed in
the prostate. STAMP family genes STAMP1/STEAP2 and STAMP2/STEAP4 have only expressed in androgen receptor-positive cells, the role of AR in STAMP family gene expression is an important question. STEAP (Six
Transmembrane Epithelial Antigens of Prostate)
is the first characterized prostate enriched six transmembrane genes,
expressed in metastatic prostate cancer samples, it is tempting to speculate
that STAMP/STEAP family genes may be involved in similar functions with a role
for both the normal biology and pathophysiology
of the prostate. Using siRNA technology in LNCaP cells
expressing STAMP genes per se, an apoptosis panel including pro-apoptotic and/or apoptotic molecules was assayed by RT-PCR. In this
research project, the prostate-specific STAMP gene family and its regulatory effects on the nuclear factor kappa B and caspase-related pathways were
characterized. Considering that the beta-actin response in the control group was high in
the immunolabeling studies, an increase in
the induction of Tumor Necrosis Factor (TNF) was detected in the signals
received with the vital proteins NFkB and akt, which were silenced by siRNA,
which means that STAMP genes potentiate vital proteins.
Share and Cite:
Gönen, C. (2022) Responses Taken by Silencing of NFkappaB, STAMP1 and STAMP2 Genes and Expression of NFkB, Act-1, p53 and p73 at -/+ TNFalpha Induced LNCaP Cells.
Journal of Cancer Therapy,
13, 685-700. doi:
10.4236/jct.2022.1312060.
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