Author(s)

Pengcheng Zhang^1,2*, Qinru Yu^1*, Ran Li¹, Yaoyao Liu¹, Tongfei Lai^1#

¹College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, China.
²Worcester-Hangzhou Joint Molecular Plant Health Laboratory, School of Science and the Environment, University of Worcester, Worcester, UK.

Small RNAs in Penicillium digitatum were identified and analyzed via transcriptome sequencing on the BGISEQ-500 platform. A total of 15 predicted miRNAs and 10718 novel siRNAs were found. Their length distribution, sequence, predicted construction, base bias, expression levels and potential targets were determined as well. Through pathway and KEGG enrichment analysis, the miRNA target genes were mostly involved in carbohydrate metabolism, transport and catabolism, translation and amino acid metabolism. The target genes involved in aflatoxin biosynthesis and proteasome had a higher rich factor value. The results will provide a theoretical foundation for understanding the developmental and pathogenic mechanisms of P. digitatum at the transcriptional level.

Penicillium digitatum, Transcriptome Sequencing, MicroRNA, Small Interfering RNA

Zhang, P. , Yu, Q. , Li, R. , Liu, Y. and Lai, T. (2022) Uncovering Small RNAs in Penicillium digitatum by Transcriptome Sequencing. American Journal of Plant Sciences, 13, 1006-1022. doi: 10.4236/ajps.2022.137067.