Author(s)

Lucien Murhula Namegabe^1,2, Aladin Ombeni Mahano¹, Serigne Omar Sarr^2,3

¹Official University of Bukavu, Faculty of Pharmaceutical Sciences and Public Health, Bukavu, Bukavu, Democratic Republic of the Congo.
²Cheikh Anta Diop University, Analytical Chemistry, and Food Sciences Laboratory, Dakar, Senegal.
³National Medecines Control Laboratory, Dakar, Senegal.

The development of the spectrofluorimetric method can be considered a promising alternative that is relatively less expensive and sufficiently reliable. In the current literature, no method for the analysis of nevirapine by spectro-fluorimetric has been reported. The proposed method is based on the transformation of naturally non-fluorescent nevirapine into a fluorescent derivative after chemical synthesis. Maximum excitation and emission wavelengths are 290 nm and 357 nm respectively. The analytical performance of the method demonstrates linearity in the concentration range 1.5 × 10^-2 and 13.5 × 10^-2 μg/mL with a correlation coefficient (r) greater than 0.999. The detection (LOD) and quantification (LOQ) limits found are 1.97 × 10^-3 μg/mL and 5.48 × 10^-3 μg/mL respectively. Recovery is achieved with 99.9% and 100.3% trueness, intra-day precision with a coefficient of variation of repeatability (CVr) of 0.99% and inter-day precision with a coefficient of variation of precision (CVR) of 1.7%. The method has been successfully applied in the analysis of 10 batches of nevirapine tablets and suspensions.

Spectrofluorimetric Method, Validation Nevirapine, Sodium Hypochlorite

Namegabe, L. , Mahano, A. and Sarr, S. (2022) Development, Validation and Application of a Spectrofluorimetric Method for the Quantification of Nevirapine in Pharmaceutical Formulations Tablets and Suspensions. American Journal of Analytical Chemistry, 13, 206-227. doi: 10.4236/ajac.2022.136015.