Author(s)

Sherif M. El-Sherbini^1*, Ahmed Kh. S. Salama², Laila A. Rashed³, Mohamed H. Nafady², Samir A. M. El-Masry¹

¹Genetic Engineering and Biotechnology Research Institute, Al-Sadat University, Al-Sadat, Egypt.
²Faculty of Applied Medical science, Misr University for Science and Technology, Giza, Egypt.
³Faculty of Medicine, Cairo University, Cairo, Egypt.

Background: Psoriasis is considered a common skin disease, marked by the production and elevation of inflammatory plaques that regularly shed scales resulting from extensive skin epithelial cell proliferation. T lymphocytes, neutrophils, and other leukocytes enter the irritated skin, resulting in epidermal keratinocyte hyperplasia, vascular hyperplasia, ectasia, and T lymphocyte, neutrophil other forms of leukocyte infiltration. Aim of the Work: the study aimed to investigate the role of IL-37 and VEGF gene expression in the pathogenesis of psoriasis in Egyptian patients. Methodology: Polymerase chain reaction techniques (PCR) were applied to detect VEGF in the skin homogenates of psoriasis patients. In addition, the ELIZA technique was applied to investigate IL-37 in skin homogenates. One hundred cases have been divided into two groups: 50 healthy volunteers as the group I healthy control; 50 psoriasis patients as group II. PCR real-time technology was assessed through extracted DNA samples for VEGF amplification in skin homogenate. Result: Our results revealed that psoriasis patients significantly had a substantial reduction in IL-37 compared to the control group (p < 0.001). However, there was a significant increase (p < 0.001) in VEGF in psoriasis patients compared to the control group. Conclusion: There is a significant inverse association between IL-37 and VEGF in psoriasis patients. Study findings revealed that IL-37 gene expression decreases while VEGF gene expression increases in psoriatic individuals. Such a measurement may be beneficial in determining the severity of the condition, as well as taking into consideration of the disease’s diagnosis.

IL-37, VEGF, Gene Expression, Psoriasis, PCR

El-Sherbini, S. , Salama, A. , Rashed, L. , Nafady, M. and El-Masry, S. (2022) Association between IL-37 and VEGF Gene Expression in Psoriasis Pathogenesis in Egyptian Population. Journal of Biosciences and Medicines, 10, 136-149. doi: 10.4236/jbm.2022.105013.