Author(s)

Mawaib Hassan Fath-Elrahman¹, Mubarak Alkarsany², Bakri Yousif Mohammed Nour³, Adam Dawoud Abakar³, Abdelrahman Eldaw Mhammed⁴, Salaheldein Gumma Elzaki⁵, Eman Osman⁶, Mubarak Elshafia⁷, Elhadi Abdalla Ahmed^4*

¹Department of Virology, National Public Health Laboratory, Khartoum, Sudan.
²Department of Medical Laboratory Sciences, Karary University, Khartoum, Sudan.
³Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan.
⁴Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan.
⁵Molecular Biology Laboratory, Department of Epidemiology, Tropical Medicine Research Institute, Khartoum, Sudan.
⁶National Tuberculosis Reference Laboratory, National Public Health Laboratory, Khartoum, Sudan.
⁷Department of Haematology and Immunohaematology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan.

Background: Prevention against human immunodeficiency virus (HIV) includes natural resistance in the population; mainly frequency of cysteine-cysteine chemokine receptor type-5 (CCR5-delta 32 mutation). By knowing the frequency of this resistance in the community, the proportion of the population susceptible to infection can be determined. This study aimed to detect for the first time the rate of CCR5-delta 32 mutation in Sudanese individuals with HIV and sex workers. Methods: Cross-sectional study was followed in the parade from 2019 through 2021, study groups were Sudanese with HIV and sex workers. Sero-negativity of sex workers was confirmed by a rapid immunochromatography test (ICT). A blood sample was targeted for DNA isolation. PCR amplification was accomplished for CCR5 wild type and CCR5-delta 32 mutation genes using specific primers. Result: Among HIV patients, males, basic education level and ages below 60 years were commonly recorded while ages below 40 years, secondary education level and single marital status were predominated in sex workers. All HIV patients were positive for CCR5 wild type and negative for CCR5-delta 32 genotype. The sex workers group showed a frequency of 3.5% (97/200) for homozygous CCR5-delta 32 mutation. Conclusion: The rating of homozygous CCR5-delta 32 genotype in studied Sudanese sex workers was relatively more than other results obtained from African countries, and the mutation was significantly detected among sex workers group (P value = 0.008) when compared to the studied HIV group.

CCR5-Delta 32, HIV, Sex Workers, Rating, Sudan

Fath-Elrahman, M. , Alkarsany, M. , Nour, B. , Abakar, A. , Mhammed, A. , Elzaki, S. , Osman, E. , Elshafia, M. and Ahmed, E. (2022) Rating of CCR5-Delta 32 Homozygous Mutation in Sudanese HIV Patients and Sex Workers. World Journal of AIDS, 12, 55-64. doi: 10.4236/wja.2022.122005.