Molecular Genotyping of Human Papillomaviruses (HPV) in HIV+ and HIV− Women in Senegal ()
Affiliation(s)
1Laboratory of Molecular Biology, Military hospital of Ouakam (HMO), Dakar, Senegal.
2AIDS Program of the Senegalese Armed Forces, Dakar, Senegal.
3Service of Immunology, Department of Pharmacy, Faculty of Medicine, Pharmacy and Odonto-Stomatology of Cheikh Anta Diop University, Dakar, Senegal.
4Public Health Unit, Faculty of Medecine, Pharmacy and Odonto-Stomatology of Cheikh Anta Diop University, Dakar, Senegal.
ABSTRACT
Background: Different studies have demonstrated high prevalence of HPV infection and dysplastic lesions of the cervix in immunocompromised patient such as women living with HIV. Is this high prevalence due to a greater susceptibility to HPV infection, which is known to be frequent in its latent form in women? Objective: This study aims to identify HPV genotypes in HIV+ and HIV− women to understand HPV molecular epidemiology in Senegal. Material and Method: Endocervical samples from 331 HIV+ and HIV− women, sexually active, were collected. The molecular identification of the 28 genotypes studied (19 HPV-HR and 9 HPV-LR) was carried out after DNA extraction, by multiplex PCR with the Anyplex™ II HPV28 detection kit from Seegene on CFX96™ Bio-Rad machine. The comparisons were made by calculating the p-value and odds ratio with R Studio software (version 4.1.0). The results were considered significant if p < 0.05. Results: The general prevalence of HPV was significantly higher in HIV+ women with 78.95% vs 64.65% for HPV; 72.18% vs 57.07% for HPV-HR; 57.14% vs 34.34% for HPV-BR (p < 0.05). Among the 28 genotypes studied, all were more frequent in HIV+ patients except HPV59, HPV66, HPV68, HPV69, HPV11 and HPV26. The most frequently found genotype was HPV56 and non-vaccine genotypes were among the most frequent. Co-infection was also more frequent in HIV+ women (p < 0.001). The study of socio-demographic factors revealed that HIV+ women aged between 35 and 50, married and using contraception were significantly more infected with HPV than the same strata of HIV− women. Conclusion: Our results showed that the prevalence of HPV, HPV-HR and HPV-BR was significantly higher in HIV+ women. Non-vaccine genotypes were among the most found genotypes. Groups of HIV+ women aged between 35 and 50, married and using contraception were significantly more infected with HPV than the same groups of HIV-women.
Share and Cite:
Faye, B. , Gueye, S. , Tine, J. , Sarr, H. and Dièye, A. (2022) Molecular Genotyping of Human Papillomaviruses (HPV) in HIV+ and HIV− Women in Senegal.
American Journal of Molecular Biology,
12, 54-66. doi:
10.4236/ajmb.2022.122006.
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