Author(s)

Christopher Krauss^1,2, Chelsey Aurelus¹, Kayla Johnston¹, Joseph Hedley¹, Satyendra Banerjee¹, Sarah Wisniewski¹, Quentin Reaves¹, Khadimou Dia¹, Shenell Brown¹, Victoria Bartlet¹, Sheritta Gavin¹, Jazmine Cuffee¹, Narendra Banerjee¹, Kuldeep Rawat¹, Santosh Mandal², Zahidur Abedin³, Somiranjan Ghosh⁴, Hirendra Banerjee^1*

¹Department of Natural Sciences and Department of Health and Human Studies, Elizabeth City State University, University of NC, Elizabeth City, NC, USA.
²Department of Chemistry, Morgan State University, Baltimore, MD, USA.
³PrimBio Research Institute LLC, Exton, PA, USA.
⁴Department of Pediatrics and Child Health, Howard University Medical School, Washington DC, USA.

Rhenium compounds have shown anti-cancer properties against many different types of cancer cell lines; however, the cellular signaling mechanisms involved in the cytotoxic properties of rhenium-based compounds were never deciphered or reported. In this manuscript, we report the results of an investigation done by RNA sequencing of rhenium treated A549 lung cancer cell lines along with an untreated vehicular control, analyzed by the Ingenuity Pathway Analysis (IPA) software system to decipher the core canonical pathways involved in rhenium induced cancer cell death. A549 EMT lung cancer cell lines were treated with rhenium ligand (Tricarbonylperrhenato(bathocuproine)rhenium(I), PR7) for seven days along with vehicular control. RNA was isolated from the treated and control cells and sequenced by a commercial company (PrimBio Corporation). The RNA sequencing data was analyzed by the INGNUITY software system and the core canonical pathways involved with differential gene expression were identified. Our report is showing that there are several cellular pathways involved in inducing cell death by rhenium-based compound PR7.

Rhenium Compounds, Lung Cancer, Epithelial

Krauss, C. , Aurelus, C. , Johnston, K. , Hedley, J. , Banerjee, S. , Wisniewski, S. , Reaves, Q. , Dia, K. , Brown, S. , Bartlet, V. , Gavin, S. , Cuffee, J. , Banerjee, N. , Rawat, K. , Mandal, S. , Abedin, Z. , Ghosh, S. and Banerjee, H. (2022) A Study of Differential Gene Expression and Core Canonical Pathways Involved in Rhenium Ligand Treated Epithelial Mesenchymal Transition (EMT) Induced A549 Lung Cancer Cell Lines by INGENUITY Software System. Computational Molecular Bioscience, 12, 12-19. doi: 10.4236/cmb.2022.121002.