Author(s)

Chunli Fu¹, Jiemei Chen¹, Lifang Fang², Tao Pan^3*

¹Department of Biological Engineering, Jinan University, Guangzhou, China.
²Guangdong Langtao Science and Technology, Co., Ltd., Dongguan, China.
³Department of Optoelectronic Engineering, Jinan University, Guangzhou, China.

The identification of soy sauce adulteration can avoid fraud, and protect the rights and interests of producers and consumers. Based on two measurement models (1 mm, 10 mm), the visible and near-infrared (Vis-NIR) spectroscopy combined with standard normal variate-partial least squares-discriminant analysis (SNV-PLS-DA) was used to establish the discriminant analysis models for adulterated and brewed soy sauces. Chubang soy sauce was selected as an identification brand (negative, 70). The adulteration samples (positive, 72) were prepared by mixing Chubang soy sauce and blended soy sauce with different adulteration rates. Among them, the “blended soy sauce” sample was concocted of salt water (NaCl), monosodium glutamate (C₅H₁₀NNaO₅) and caramel color (C₆H₈O₃). The rigorous calibration-prediction-validation sample design was adopted. For the case of 1 mm, five waveband models (visible, short-NIR, long-NIR, whole NIR and whole scanning regions) were established respectively; in the case of 10 mm, three waveband models (visible, short-NIR and visible-short-NIR regions) for unsaturated absorption were also established respectively. In independent validation, the models of all wavebands in the cases of 1 mm and 10 mm have achieved good discrimination effects. For the case of 1 mm, the visible model achieved the optimal validation effect, the validation recognition-accuracy rate (RAR_V) was 99.6%; while in the case of 10 mm, both the visible and visible-short-NIR models achieved the optimal validation effect (RAR_V = 100%). The detection method does not require reagents and is fast and simple, which is easy to promote the application. The results can provide valuable reference for designing small dedicated spectrometers with different measurement modals and different spectral regions.

Visible and Near-Infrared Spectroscopy, Soy Sauce, Adulteration Identification, Partial Least Squares-Discriminant Analysis, Standard Normal Variate

Fu, C. , Chen, J. , Fang, L. and Pan, T. (2022) Visible and Near-Infrared Spectroscopic Discriminant Analysis Applied to Identification of Soy Sauce Adulteration. American Journal of Analytical Chemistry, 13, 51-62. doi: 10.4236/ajac.2022.132005.