Author(s)

Fidan Huseynova^1,2, Alamdar Mammadov², Irada Huseynova², Frédéric Cuisinier¹, Véronique Barragan-Montero¹

¹LBN, University Montpellier, Montpellier, France.
²Institute Molecular Biology and Biotechnology of ANAS, Baku, Azerbaijan.

Insulin, an old but still actual molecule, has many roles in various cellular processes including bone formation and also angiogenesis. Insulin effects on different cell types were investigated, and we intended to check its effect on dental pulp cells (DPC) during osteoblast differentiation. First, bone differentiation ability of isolated dental pulp cells was assessed by alizarin red (AR) staining. Insulin increased dental pulp cell proliferation after 25 hours of culture. It increased mRNA expression of osteogenic markers such as Col1, RunX2, ALP, Osc, Mef2C and expression of genes involved in TGF b pathway such as Smad3, TSP1, VEGF at different time points.

Dental Pulp Cells, Gene Expression, Osteodifferentiation, Insulin

Huseynova, F. , Mammadov, A. , Huseynova, I. , Cuisinier, F. and Barragan-Montero, V. (2022) Insulin Effect on Gene Expression of Dental Pulp Cell during Osteodifferentiation. Journal of Biosciences and Medicines, 10, 99-107. doi: 10.4236/jbm.2022.101009.