Author(s)

Ariba Khan¹, Farah Naz Talpur^1*, Muhammad Iqbal Bhanger², Syed Ghulam Musharraf², Hassan Imran Afridi¹

¹National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan.
²Hussain Ebrahim Jamal Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.

To attain maximum recovery of useful compounds from slaughterhouse waste (Suet, Tongue, Pancreas) of selected ruminant (cow, goat, lamb, and bull), the fat extraction efficiency of popular methods was compared along with fatty acid (FAs) composition. Four selected methods including Soxhlet (SOX), acid hydrolysis, Bligh & dyer (B&D), and Folch (FOL) were assessed. After methylation, extracted lipids were analyzed by Gas chromatography for FA composition. Data indicated that all selected methods were significantly (p < 0.05) different from each other, particularly higher differences were noticed for low lipid-containing products (Tongue, Pancreas) as well as their respective FA Composition. Based on Analysis of Variance and Principal component analysis, the effective method for lipid and FA Composition analysis was the FOL method. The Soxhlet method was only effective for samples with high-fat content i.e., suet, while the B&D method gave comparatively low lipid content in analyzed samples. Hence based on the results, excellent fat and fatty acid extraction was achieved with the FOL method.

Fatty Acid, Slaughterhouse Waste, Animal by-Products, Extraction Methods, Lipid Analysis, Gas Chromatography

Khan, A. , Talpur, F. , Bhanger, M. , Musharraf, S. and Afridi, H. (2021) Extraction of Fat and Fatty Acid Composition from Slaughterhouse Waste by Evaluating Conventional Analytical Methods. American Journal of Analytical Chemistry, 12, 202-225. doi: 10.4236/ajac.2021.125013.