Author(s)

Takuya Kuraoka¹, Takayoshi Yamada¹, Yuki Takatsutsumi¹, Yukiko Ogawa², Hidemitsu Kobayashi^1*

¹Divisions of Microbiology, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan.
²Divisions of Infection Control and Prevention, Department of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan.

On two dimensional maps of ¹H-¹³C correlation spectroscopy (H-C COSY) analysis for the mannan of Candida tropicalis, nine cross peaks of anomeric proton and carbon were useful for the purpose of obtaining information on the chemical structure of this molecule. Namely, the mannans was comb-like structure constructed with the linear α-1,6-linked polymannnosyl backbone and several oligomannnosyl side chains composed of α-1,2-, α-1,3-, and β-1,2-linkages. Therefore, in the structural investigation of comb-like mannan, two-dimensional H-C COSY analysis is as useful as two-dimensional nuclear Hartmann-Hahn (HOHAHA) analysis.

Candida tropicalis, Cell Wall Mannan, Comb-Like Structure, ¹H-¹³C Correlation Spectroscopy, Anomeric Carbon Chemical Shift, α-1, 3-Linked Mannose

Kuraoka, T. , Yamada, T. , Takatsutsumi, Y. , Ogawa, Y. and Kobayashi, H. (2021) Anomeric Proton and Carbon (H1-C1) NMR Chemical Shifts of Antigenic Mannans Obtained from Pathogenic Yeast Candida tropicalis. Advances in Microbiology, 11, 296-301. doi: 10.4236/aim.2021.115022.