Genetic Variation among Seedling of Pumpkins Genotypes through SDS-Page - American Journal of Plant Sciences

AJPS > Vol.12 No.5, May 2021

American Journal of Plant Sciences

Volume 12, Issue 5 (May 2021)

ISSN Print: 2158-2742 ISSN Online: 2158-2750

Google-based Impact Factor: 1.20 Citations h5-index & Ranking

Genetic Variation among Seedling of Pumpkins Genotypes through SDS-Page ()

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DOI: 10.4236/ajps.2021.125048 294 Downloads 1,281 Views Citations

Author(s)

Muhammad Ikram^1*, Niaz Ali¹, Muhammad Ishaq¹, Muhammad Romman², Yasir Islam¹, Naeem Khan^3*

Affiliation(s)

¹Department of Botany, Hazara University Mansehra, Khyber Pakhtunkhwa, Pakistan.
²Department of Botany, University of Chitral, Khyber Pakhtunkhwa, Pakistan.
³Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

ABSTRACT

Genetic diversity in 30 genotypes of Pumpkins was collected from unexplored mountainous areas of Khyber Pakhtunkhwa, Pakistan was investigated through biochemical characterization. For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis was carried out. The seed proteins were resolved on 7.5% and 15% polyacrylamide gel. A total of 35% genetic disagreement was observed in the collected lines with linkage distances ranging from 0.00 - 0.83 (percent disagreement). Similarly, cluster analysis sorted total germplasm on the basis of 12 bands (total bands) into eight clusters. Present study revealed a considerable amount of genetic diversity explored in pumpkin germplasm, Cluster analysis exhibited moderate level of genetic diversity; to broaden the gene pool. Further collection of the important germplasm is needed to be used in the development of improved cultivars with respect to quality and quantity.

KEYWORDS

Genetic Diversity, SDS-PAGE, Pumpkin, Cluster Analysis

Share and Cite:

Ikram, M. , Ali, N. , Ishaq, M. , Romman, M. , Islam, Y. and Khan, N. (2021) Genetic Variation among Seedling of Pumpkins Genotypes through SDS-Page. American Journal of Plant Sciences, 12, 711-719. doi: 10.4236/ajps.2021.125048.

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