Biotyping of Multidrug Resistant Campylobacter jejuni from Poultry and Humans in Northern Region of Ghana ()
Affiliation(s)
1Department of Clinical Microbiology, School of Medicine and Health Sciences, University for Development Studies, P. O. Box TL 1350, Tamale, Ghana.
2Department of Biotechnology, Faculty of Agriculture, University for Development Studies, P. O. Box TL 1882, Tamale, Ghana.
ABSTRACT
Thermophilic Campylobacters are zoonotic bacteria which are universally famous for causing enteritis in
humans. They are normally found as commensals in the digestive tract of food
animals with poultry being a major reservoir
of the pathogenic species. This study highlighted the presence of Campylobacter in poultry (commercial and
domestic) and humans (patients and asymptomatic individuals) and characterized
strains by biotyping and susceptibility test in the Northern region of Ghana
where animal husbandry is commonly practiced but zoonotic studies are limited.
A total of 1087 specimens from stools of humans and cloacal swabs of poultry
were screened from 25th October, 2017 to 7th May, 2019. Samples were cultured
on modified charcoal-cefoperazone-deoxycholate agar and isolates identified
using standard microbiological procedures and Lior Biotyping, while the
antibiogram of isolates was determined by the Kirby-Bauer disk diffusion method. The frequency of Campylobacter was 43.1% and 12.9%
respectively in poultry and humans. Campylobacter
jejuni were recovered from 84% of commercial birds and 64% of domestic
birds and in humans significantly fewer strains were observed in patients than asymptomatic individuals (p < 0.05). Biotype
distribution revealed C. jejuni biotype
I prevalence in domestic birds, patients and asymptomatic individuals whereas
Bioytype II was largely found in commercial poultry. All isolated strains of Campylobacter were resistant to
tetracycline and 69.4% of Campylobacter
jejuni strains were resistant to erythromycin. Imipenem and the
aminoglycosides were relatively effective as resistance of 10% and below 20%
were respectively obtained. None of the endorsed treatment drugs (erythromycin,
ciprofloxacin, and tetracycline) can be admitted
in this region due to common resistance found among strains against
these agents.
Share and Cite:
Karikari, A. , Saba, C. and Kpordze, S. (2021) Biotyping of Multidrug Resistant
Campylobacter jejuni from Poultry and Humans in Northern Region of Ghana.
Open Journal of Medical Microbiology,
11, 18-31. doi:
10.4236/ojmm.2021.111002.