Author(s)

Muhammad Ikram^1*, Niaz Ali¹, Gul Jan², Farzana Gul Jan², Muhammad Romman³, Muhammad Ishaq¹, Yasir Islam¹, Naeem Khan^4*

¹Department of Botany, Hazara University, Mansehra, Pakistan.
²Department of Botany, Abdul Wali Khan University Mardan, Pakistan.
³Department of Botany, University of Chitral, Chitral, Pakistan.
⁴Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

Present study aims to evaluate antimicrobial and antioxidant activities of the crude methanolic extract and different fractions of the Epilobium roseum (Schreb). The extract and fractions were used against pathogenic bacteria (Bacillus subtilis, Bacillus atrophaeus, Klebsiella pneumonia and Pseudomonas aeruginosa) and fungal strains (Aspergillus niger and Aspergillus flavus). The methanolic extract and their sub fraction n-hexane showed a prominent inhibition zone against all bacterial strains but inactive against fungal strains. The various extracts of Epilobium roseum (Schreb) from various parts were tested for their antioxidant activity by 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assay. The IC₅₀ of the stem and root of methanolic extract and their sub fraction n-hexane showed best activity ranged between 22.73 ± 6.92, 21.49 ± 6.26 and 14.94 ± 3.54, 13.92 ± 1.04 μg/ml compared to another fraction. The results support that Epilobium roseum can be used as antimicrobial and antioxidant agents. The results support the present study that Epilobium roseum (Schreb) has a potential source of natural antibacterial, antifungal and antioxidant potentials.

Methanolic Extract, Epilobium roseum, DPPH, n-Hexan, Antioxidant Activity

Ikram, M. , Ali, N. , Jan, G. , Jan, F. , Romman, M. , Ishaq, M. , Islam, Y. and Khan, N. (2021) Antimicrobial and Antioxidant Activities of Methanolic Extract and Fractions of Epilobium roseum (Schreb.) against Bacterial Strains. American Journal of Plant Sciences, 12, 275-284. doi: 10.4236/ajps.2021.123017.