Author(s)

Chukwudi Umego¹, Valentine Otang Ntui^1,2, Effiom Eyo Ita¹, Charles Opara¹, Edak Aniedi Uyoh^1*

¹Department of Genetics and Biotechnology, University of Calabar, Calabar, Nigeria.
²International Institute for Tropical Agriculture, Nairobi, Kenya.

Drought and salinity are the most widespread soil problems, posing a big threat to food security in rice growing regions. The present study evaluated the performance of eleven rice genotypes using morphological and physiological parameters, under induced drought and salinity conditions. The seedlings were raised in 5 kg of homogenous soil in plastic bags in the greenhouse. For the drought experiment, each bag was watered with 200 ml of water twice daily until plants reached the five-leaf stage when watering was suspended for 2 weeks for the drought stressed plants but not suspended for the control plants. The experiment was a 2 × 11 factorial and the set up was arranged using the completely randomized design with three replications. Data were taken on Plant height, Number of tillers, leaf length, Number of green leaves, Number of dead leaves, Leaf rolling score (LRS) and Rate of water loss. The salinity experiment was set up in a similar manner except that the plants were irrigated twice a day for 2 weeks with 200 ml of treatment solution containing either 0 mM NaCl or 75 mM and data were collected on plant height, number of tillers, shoot fresh weight, shoot dry weight, Na⁺ and K⁺ concentrations, relative water content and chlorophyll content. Data from both experiments were subjected to Analysis of variance test using the GenStat software 10^th edition and the means separated using least significant difference test. Individual stress response index (ISRI) was calculated for each parameter and the means used in grouping the varieties. Of the genotypes evaluated, four (FARO 44, NERICA 2, NERICA 8 and NERICA 5) were identified as tolerant, two (NERICA 4 and FARO 57) as moderately tolerant, while the rest were found to be sensitive to drought. Equally, two varieties (FARO 44 and RAM 137) stood out in the salinity screening as tolerant varieties, five were moderately tolerant while four (FARO 64, FARO 52, NERICA 2 and FARO 55) were clearly susceptible. FARO 44 is the only genotype that showed tolerance to both drought and salinity. The identified drought and salinity tolerant rice genotypes from this study can be recommended as genetic sources for future breeding programs for drought and salinity tolerance in rice.

Oryza sativa, Salinity Tolerance, Drought Tolerance, Morpho-Physiological Parameters, Stress Response Indices

Umego, C. , Ntui, V. , Ita, E. , Opara, C. and Uyoh, E. (2020) Screening of Rice Accessions for Tolerance to Drought and Salt Stress Using Morphological and Physiological Parameters. American Journal of Plant Sciences, 11, 2080-2102. doi: 10.4236/ajps.2020.1112147.