Author(s)

J. Kim^1*#, K. Chung^2*, L. K. Fuerniss³, B. J. Johnson³

¹Department of Animal Science & Food Science and Human Nutrition, Michigan State University, East Lansing, MI, USA.
²Department of Beef Science, Korean National College of Agriculture and Fisheries, Jeollabuk-do, South Korea.
³Department of Animal and Food Science, Texas Tech University, Lubbock, TX, USA.

Numerous physiological and pathological processes are controlled by free fatty acids, which act as signaling molecules in mammals. We hypothesized that oleic acid (Ole) might stimulate the formation of satellite-cell-derived intramuscular adipose tissue. The role of Ole as a ligand of G-protein-coupled receptor 43 (GPR43) was previously identified. Thus, the objective of the current study was to determine the effect of Ole on GPR43 and factors related to the adipogenic differentiation of bovine satellite cells (BSC). Treatments of 100 μM and 500 μM Ole tended to induce greater (P < 0.10) mRNA expression of CCAAT/enhancer-binding protein β (C/EBPβ) compared to all other doses. The mRNA abundance of peroxisome proliferator-activated receptor γ (PPARγ) was not altered (P > 0.10) by treatment. The addition of 100 μM and 500 μM of Ole upregulated (P < 0.05) GPR43 mRNA expression. Protein level of GPR43 was increased (P < 0.05) by 100 μM of Ole treatments. Addition of Ole to BSC cultures induced transformation of myogenic cells into adipocyte-like cells that formed cytoplasmic lipid droplets. Increased expression of C/EBPβ in response to Ole might suppress myogenic differentiation. After the treatment of cells with Ole, increased expression of GPR43 could lead to phosphorylation of 5’ AMP-activated protein kinase α (AMPKα). Altogether, the results indicated that increased Ole might stimulate adipose tissue accumulation within the skeletal muscle of cattle by promoting adipogenic differentiation and activation of GPR43 in satellite cells.

Bovine Satellite Cells, GPR43, Intramuscular Adipose Tissue, Oleic Acid

Kim, J. , Chung, K. , Fuerniss, L. and Johnson, B. (2020) Oleic acid Stimulates the Formation of Adipocyte-Like Cells from Bovine Satellite Cells via G-Protein Coupled Receptor 43 and CCAAT/Enhancer-Binding Protein Beta. Open Journal of Animal Sciences, 10, 649-664. doi: 10.4236/ojas.2020.104042.