This paper discusses the sporo pollen assemblage, evolution of sedimentary environment, sedimentary facies and the lithotope characteristics revealed by boreholes since pliocene epoch by carrying out ¹⁴C dating, sporo pollen and micro-paleontological analysis of sediments in borehole BK01 (depth 237.80 m) in the southern flank of the Yangtze River Delta Plain. According to the results of this study, there are 17 species of foraminifera that have been identified as 11 genera, including 16 benthic foraminifera and 1 planktonic foraminifera；8 species of Ostracoda that have been identified as 8 genera; sporo pollen analysis shows that there are 37 types, including 18 species of woody plant pollen, 11 species of herbaceous plant pollen, 8 species of fern spores, of which, in the sporo pollen assemblage, woody plants have the highest content (about 85.1%), ferns spores have about 8.7%, and herbaceous plants have only 6.3%. Sedimentary environment records can be divided into 18 sporo pollen assemblage zones according to borehole lithology, sporo pollen assemblage and micropaleontology analysis. The results show that the BK01 bore strata from bottom to top can be divided into carbonaceous mudstone (Ech), Jiaxing Formation (N-Qp₁j), Qiangang Formation (Qp₂q) Dongpu Formation (Qp₃d), Ningbo Formation (Qp₃n) and Zhenhai Formation (Qhzh).