Recently, the life of worldwide human beings has been endangering by the spreading of pneu- monia-causing virus, such as Coronavirus, COVID-19, and H1N1. To develop effective drugs against Coronavirus, knowledge of protein subcellular localization is prerequisite. In 2019, a predictor called “pLoc_bal-mEuk” was developed for identifying the subcellular localization of eukaryotic proteins. Its predicted results are significantly better than its counterparts, particularly for those proteins that may simultaneously occur or move between two or more subcellular location sites. However, more efforts are definitely needed to further improve its power since pLoc_bal-mEuk was still not trained by a “deep learning”, a very powerful technique developed recently. The present study was devoted to incorporating the “deep- learning” technique and developed a new predictor called “pLoc_Deep-mEuk”. The global absolute true rate achieved by the new predictor is over 81% and its local accuracy is over 90%. Both are overwhelmingly superior to its counterparts. Moreover, a user-friendly web- server for the new predictor has been well established at http://www.jci-bioinfo.cn/pLoc_Deep-mEuk/, by which the majority of experimental scientists can easily get their desired data.