Special Issue on The Application of Phosphorylation in Biological Chemistry
the addition of a phosphate group to a protein or other organic molecule.
Phosphorylation turns many protein enzymes on and off, thereby altering their
function and activity. Protein phosphorylation is one type of
post-translational modification. Protein phosphorylation in particular plays a
significant role in a wide range of cellular processes. Its prominent role in
biochemistry is the subject of a very large body of research.
characterization of the sites of phosphorylation is very difficult, and the
quantitation of protein phosphorylation by mass spectrometry requires isotopic
internal standard approaches. A relative quantitation can be obtained with a
variety of differential isotope labeling technologies. There are also several
quantitative protein phosphorylation methods, including fluorescence
immunoassays, Microscale Thermophoresis, FRET, TRF, fluorescence polarization,
fluorescence-quenching, mobility shift, bead-based detection, and cell-based
formats. Antibodies can be used as powerful tools to detect whether a protein
is phosphorylated at a particular site. Antibodies bind to and detect
phosphorylation-induced conformational changes in the protein. Such antibodies
are called phospho-specific antibodies; hundreds of such antibodies are now
available.They are becoming critical reagents both for basic research and for
In this special
issue, we intend to invite front-line researchers and authors to submit
original research and review articles on exploring The Application of
Phosphorylation in Biological Chemistry.
Authors should read
over the journal’s Author’s Guidelines carefully before
submission, Prospective authors should submit an electronic copy of their
complete manuscript through the journal Paper Submission System.
Please kindly notice that the “Special Issue’’ under your
manuscript title is supposed to be specified and the research field “Special Issue — The Application of
Phosphorylation in Biological Chemistry” should be
chosen during your submission.
According to the
Prof. Gary W. Black; Northumbria University, UK
For further questions or inquiries
Editorial Assistant at