Author(s)

Priyanka Prathipati^1*, Cristian Rodriguez-Aguayo^2,3, Brian L Walton¹, Anil K. Sood^3,4,5, Jamieson Greaver⁶, Christopher F. Janssen⁶, Gabriel Lopez-Berestein^2,3,4

¹Department of Advanced Cardiopulmonary Therapies and Transplantation, Center for Advanced Heart Failure, University of Texas Health Science Center at Houston, Houston, TX, USA.
²Department of Experimental Therapeutics, University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
³The Center for RNA Interference and Non-Coding RNA, University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
⁴Department of Cancer Biology, University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
⁵Department of Gynecology Oncology & Reproductive Medicine, University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
⁶Interdisciplinary Research, Center for Laboratory Animal Medicine and Care, University of Texas Health Science Center at Houston, Houston, TX, USA.

Liposomes effectively transport fatty proteins to targeted tissues. Laboratory experiments use multiple methods to administer liposomes, but comparison of these methods is not available. In this retrospective study, we characterized and compared four intravenous administration routes (tail vein, jugular catheter, femoral vein and percutaneous retro-orbital injections) in murine models. ApoE^-/- mice were used to compare administration routes. Results indicate that the jugular catheter route delivered the highest amount of liposomes to tissues due to longer period of injections compared to other routes; however, this route failed to remain patent for 8/10 animals. Delivery via tail vein, femoral vein and percutaneous retro-orbital injections resulted in similar accumulation in the organs. When including technical difficulty and expense, percutaneous retro-orbital injections of liposomes are the most convenient and efficacious approach.

Femoral Vein, Jugular Catheter, Liposomes, Retro-Orbital, Tail Vein

Prathipati, P. , Rodriguez-Aguayo, C. , Walton, B. , Sood, A. , Greaver, J. , Janssen, C. and Lopez-Berestein, G. (2020) A Retrospective Analysis for Different Routes of Administration in Mice-Percutaneous Retro-Orbital, Jugular Catheter, Tail Vein and Femoral Cut Down Injections. Journal of Biosciences and Medicines, 8, 131-141. doi: 10.4236/jbm.2020.89011.