Model of Sterile Intestine of Mices with Normal Microfloras: Histological and Ultrastructural Visualization Formation Kyst Organ-Like Intestine for the Study of Interaction of Pathogen Agents with the Host Sterile Intestine

Karlen Hovnanyan

Institute of Molecular Biology NAS RA, Yerevan, Armenia.

DOI: 10.4236/oalib.1100431                

Abstract

For obtainment of the isolated sterile intestine, inbred lines of mice C3HA, CBA, C57BL/6, BalB/c are used at same time; it is carried out transplantation of small and large intestines of embryons corresponding lines of mice subcutaneously in adult singen mice. In capacite of test objects we make use of cultures of Salmonella typhimurium. The side of organ like kysts of isolated sterile of small and large intestines’ models on the histological and transmission electron-microscopic (TEM) preparations have their own typical composition. In case of electron-microscopic examination of steril small intestine they were revealed microvillus of epithelium, Salmonella typhimurium in lining epithelium and phagosoma of lymphoid cells of lamina propria of mucose.

Keywords

Sterile Intestine, Model, Electron Microscopy, Ultrastructura, Parasit-Host

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Wostmann, B.S. (1996) Germfree and Gnotobiotic Animal Models: Background and Applications. CRC Press, Inc., Boca Raton, 208.
[2]	Reyniers, JA (1959) Germfree Vertebrates: Present Status. Annals of the New York Academy of Sciences, 78, 3-16. http://dx.doi.org/10.1111/j.1749-6632.1959.tb53091.x
[3]	Svedsen, P. and Hau, J. (1994) Handbook of Laboratory Animal Science. Vol. II: Animal Models, CRC Press, Inc., Boca Raton, 224.
[4]	Astafyev B.A., Yarotsckiim, L.S. and Lebedeva, M.N. (1989) Experimental Models of Parasitoses in Biology and Medicine. Nauka Press, Moscow, 279.
[5]	Voyno-Yasenecki, M.V. (1981) Biology and Pathology of Infections Processings. “Medicina” Press, St.-Petersburg, 207.
[6]	Metchnikoff, E. (1908) études sur la flore intestinale. Annales de l’Institut Pasteur, 22, 929-955.
[7]	Gostev, V.V., Bondarenko, V.M., Marchenko, V.G. and Klitsunova, N.V. (1986) Ultrastructural Aspects of Process Interaction Salmonella typhi with Cages of line L929. The Journal of Microbiology, 5, 34-37.
[8]	Chakhava, O.V., Mountain, Е. and Ruban, S.Z. (1982) Microbiological and Immunological of Bases of Gnotobiology. Medicina Press, Moscow, 160.
[9]	Ito, S. and Karnovsky, M.J. (1968) Formaldehyde-Glutaraldehyde Fixatives Containing Trinitrocompounds. The Journal of Cell Biology, 39, 168A-169A.
[10]	Venable, J.H. and Coggeshall, R. (1965) A Simplified Lead Citrate Stain For Use in Electron Microscopy. Journal of Cell Biology, 25, 407-408. http://dx.doi.org/10.1083/jcb.25.2.407
[11]	Ghukasyan, G.B., Tonoyan, I.A. and Tendetnik, Y.Y. (1983) Primary and Secondary Localisation of Salmonellas in Experiment. Proceeding of Theses of Reports of 3rd Republican Congress of Epidemiologists, Microbiologists and Parasitologists, Yerevan, 20-22 September 1983, 35-36.