Heat Shock Protein 70 Gene Transfection Protects Rat Myocardium Cell Against Anoxia-Reoxygeneration Injury

DOI:                 

Abstract

The cultured primary neonatal rat myocardiocytes with an acute myocardial A/R injury model and the HS-treated rat myocardiocyte model were used. Three-day cultured myocardiocytes were randomly divided into four groups (n=8): control group, A/R group, HS+A/R group and pCDNA HSP70 +A/R group. A liposome-coated HSP70 pCDNA plasmid was transfected into the primary neonatal rat myocardiocytes; HSP70 mRNA and its protein were confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting. The cell viability was assayed by monotetra-zolium (MTT) and the lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) activity of cells during incuba-tion and the changes in cells ultrastructure were examined. NF-κB activity in the primary neonatal rat myocardiocytes was measured with flow cytometry.

Keywords

gene transfection, HSP70 gene, NF-κB, cardiac myocyte, anoxia-reoxygeneration injury

Conflicts of Interest

The authors declare no conflicts of interest.

References